Detailed information of TA system TA213114

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3763666..3764345
• Replicon: chromosome
• Accession: NZ_CP081701
• Organism: Escherichia coli strain AH02

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O02_RS18715
• Protein ID: WP_000854680.1
• Coordinates: 3764004..3764345 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O02_RS18710
• Protein ID: WP_000070396.1
• Coordinates: 3763666..3763983 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O02_RS18665 (3759104)	3759104..3759925	+	822	WP_000197387.1	DUF932 domain-containing protein	-
J6O02_RS18670 (3760142)	3760142..3760843	+	702	Protein_3654	WYL domain-containing protein	-
J6O02_RS18675 (3760884)	3760884..3761120	+	237	WP_001144031.1	protein YpjK	-
J6O02_RS18680 (3761120)	3761120..3761563	+	444	WP_000649865.1	hypothetical protein	-
J6O02_RS18685 (3761586)	3761586..3762053	+	468	WP_001385283.1	protein YkfB	-
J6O02_RS18690 (3762130)	3762130..3762369	+	240	WP_000194654.1	DUF905 family protein	-
J6O02_RS18695 (3762467)	3762467..3762925	+	459	Protein_3659	antirestriction protein	-
J6O02_RS18700 (3762941)	3762941..3763417	+	477	WP_000811693.1	RadC family protein	-
J6O02_RS18705 (3763426)	3763426..3763647	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O02_RS18710 (3763666)	3763666..3763983	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O02_RS18715 (3764004)	3764004..3764345	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O02_RS18720 (3764588)	3764588..3765285	+	698	WP_223566534.1	IS1-like element IS1A family transposase	-
J6O02_RS18725 (3765793)	3765793..3766776	-	984	WP_223571321.1	anti-phage protein KwaB	-
J6O02_RS18730 (3766786)	3766786..3767388	-	603	WP_023565260.1	anti-phage protein KwaA	-
J6O02_RS18735 (3767995)	3767995..3768267	-	273	WP_000783295.1	ogr/Delta-like zinc finger family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3759104..3836430	77326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T213114 WP_000854680.1 NZ_CP081701:3764004-3764345 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T213114 NZ_CP107463:329318-329686 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT213114 WP_000070396.1 NZ_CP081701:3763666-3763983 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT213114 NZ_CP107463:329100-329321 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7