Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2600086..2600724 Replicon chromosome
Accession NZ_CP081701
Organism Escherichia coli strain AH02

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J6O02_RS12950 Protein ID WP_000813794.1
Coordinates 2600548..2600724 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J6O02_RS12945 Protein ID WP_001270286.1
Coordinates 2600086..2600502 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O02_RS12925 (2595238) 2595238..2596179 - 942 WP_001251313.1 ABC transporter permease -
J6O02_RS12930 (2596180) 2596180..2597193 - 1014 WP_057108537.1 ABC transporter ATP-binding protein -
J6O02_RS12935 (2597211) 2597211..2598356 - 1146 Protein_2535 ABC transporter substrate-binding protein -
J6O02_RS12940 (2598601) 2598601..2600007 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J6O02_RS12945 (2600086) 2600086..2600502 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J6O02_RS12950 (2600548) 2600548..2600724 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J6O02_RS12955 (2600946) 2600946..2601176 + 231 WP_000494244.1 YncJ family protein -
J6O02_RS12960 (2601268) 2601268..2603229 - 1962 WP_001488038.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J6O02_RS12965 (2603302) 2603302..2603838 - 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
J6O02_RS12970 (2603930) 2603930..2605105 + 1176 WP_057108539.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T213112 WP_000813794.1 NZ_CP081701:c2600724-2600548 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T213112 NZ_CP107461:c6004-5690 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT213112 WP_001270286.1 NZ_CP081701:c2600502-2600086 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT213112 NZ_CP107461:c6270-5992 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References