Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2451180..2451916 Replicon chromosome
Accession NZ_CP081701
Organism Escherichia coli strain AH02

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag J6O02_RS12190 Protein ID WP_003026803.1
Coordinates 2451180..2451662 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag J6O02_RS12195 Protein ID WP_003026799.1
Coordinates 2451650..2451916 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O02_RS12180 (2446805) 2446805..2447266 - 462 WP_000593665.1 response regulator -
J6O02_RS12185 (2447259) 2447259..2450621 - 3363 WP_029404079.1 ATP-binding protein -
J6O02_RS12190 (2451180) 2451180..2451662 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
J6O02_RS12195 (2451650) 2451650..2451916 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
J6O02_RS12200 (2452361) 2452361..2452591 - 231 WP_004206660.1 hypothetical protein -
J6O02_RS12205 (2452605) 2452605..2452808 - 204 WP_004213596.1 HHA domain-containing protein -
J6O02_RS12210 (2452869) 2452869..2453360 - 492 WP_015065591.1 hypothetical protein -
J6O02_RS12215 (2453494) 2453494..2454528 - 1035 WP_015065590.1 hypothetical protein -
J6O02_RS12220 (2454830) 2454830..2455834 - 1005 WP_022542389.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T213111 WP_003026803.1 NZ_CP081701:c2451662-2451180 [Escherichia coli]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T213111 NZ_CP107460:c91481-91065 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT213111 WP_003026799.1 NZ_CP081701:c2451916-2451650 [Escherichia coli]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT213111 NZ_CP107460:c91708-91478 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References