Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 949944..950598 Replicon chromosome
Accession NZ_CP081701
Organism Escherichia coli strain AH02

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag J6O02_RS04855 Protein ID WP_000244777.1
Coordinates 950191..950598 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J6O02_RS04850 Protein ID WP_000354046.1
Coordinates 949944..950210 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O02_RS04825 (945113) 945113..945856 + 744 WP_000951948.1 SDR family oxidoreductase -
J6O02_RS04830 (945913) 945913..947346 - 1434 WP_223566651.1 6-phospho-beta-glucosidase BglA -
J6O02_RS04835 (947391) 947391..947702 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
J6O02_RS04840 (947866) 947866..948525 + 660 WP_000250274.1 hemolysin III family protein -
J6O02_RS04845 (948721) 948721..949701 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
J6O02_RS04850 (949944) 949944..950210 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J6O02_RS04855 (950191) 950191..950598 + 408 WP_000244777.1 protein YgfX Toxin
J6O02_RS04860 (950638) 950638..951159 - 522 WP_001055874.1 flavodoxin FldB -
J6O02_RS04865 (951271) 951271..952167 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J6O02_RS04870 (952192) 952192..952902 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J6O02_RS04875 (952908) 952908..954641 + 1734 WP_033816119.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T213099 WP_000244777.1 NZ_CP081701:950191-950598 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T213099 NZ_CP107458:877170-877577 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT213099 WP_000354046.1 NZ_CP081701:949944-950210 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT213099 NZ_CP107458:876923-877189 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References