Detailed information of TA system TA213098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 870091..871659
• Replicon: chromosome
• Accession: NZ_CP081701
• Organism: Escherichia coli strain AH02

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J6O02_RS04430
• Protein ID: WP_000783857.1
• Coordinates: 870346..871659 (+)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A859R2S1
• Locus tag: J6O02_RS04425
• Protein ID: WP_039272366.1
• Coordinates: 870091..870342 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O02_RS04390 (865317)	865317..865433	+	117	WP_000991086.1	hypothetical protein	-
J6O02_RS04395 (865746)	865746..866471	+	726	WP_001553093.1	hypothetical protein	-
J6O02_RS04400 (866527)	866527..866979	+	453	WP_079778891.1	hypothetical protein	-
J6O02_RS04405 (866995)	866995..867597	-	603	WP_039272372.1	hypothetical protein	-
J6O02_RS04410 (867858)	867858..868139	+	282	WP_000575656.1	hypothetical protein	-
J6O02_RS04415 (868158)	868158..868721	+	564	WP_223571365.1	hypothetical protein	-
J6O02_RS04420 (868724)	868724..869728	+	1005	WP_001553091.1	tyrosine-type recombinase/integrase	-
J6O02_RS04425 (870091)	870091..870342	+	252	WP_039272366.1	helix-turn-helix domain-containing protein	Antitoxin
J6O02_RS04430 (870346)	870346..871659	+	1314	WP_000783857.1	type II toxin-antitoxin system HipA family toxin	Toxin
J6O02_RS04435 (871656)	871656..872150	+	495	WP_039272364.1	hypothetical protein	-
J6O02_RS04440 (872326)	872326..873264	-	939	WP_000395773.1	hypothetical protein	-
J6O02_RS04445 (873508)	873508..875103	+	1596	WP_239605188.1	PAAR domain-containing protein	-
J6O02_RS04450 (875301)	875301..875858	-	558	WP_003100847.1	recombinase family protein	-
J6O02_RS04455 (875852)	875852..876223	-	372	WP_003100853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	849853..879854	30001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-64)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 48209.48 Da
• Isoelectric Point: 6.4189

>T213098 WP_000783857.1 NZ_CP081701:870346-871659 [Escherichia coli]
MKMQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQAR
FRVETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTA
LLWYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWS
KSGEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEP
ASSFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEV
VATVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Nucleotide

• Length: 1314 bp

>T213098 NZ_CP107458:353992-354339 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9369.79 Da
• Isoelectric Point: 9.8930

>AT213098 WP_039272366.1 NZ_CP081701:870091-870342 [Escherichia coli]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Nucleotide

• Length: 252 bp

>AT213098 NZ_CP107458:354339-354638 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A859R2S1