Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4184707..4185302 Replicon chromosome
Accession NZ_CP081698
Organism Escherichia coli strain CT02

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag J6N91_RS20260 Protein ID WP_000239579.1
Coordinates 4184707..4185057 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag J6N91_RS20265 Protein ID WP_001223208.1
Coordinates 4185051..4185302 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6N91_RS20240 (4180156) 4180156..4181178 - 1023 WP_001295197.1 ABC transporter permease -
J6N91_RS20245 (4181192) 4181192..4182694 - 1503 WP_000205800.1 sugar ABC transporter ATP-binding protein -
J6N91_RS20250 (4182834) 4182834..4183790 - 957 WP_000265942.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
J6N91_RS20255 (4184100) 4184100..4184630 + 531 WP_000055072.1 inorganic diphosphatase -
J6N91_RS20260 (4184707) 4184707..4185057 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
J6N91_RS20265 (4185051) 4185051..4185302 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
J6N91_RS20270 (4185514) 4185514..4185855 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
J6N91_RS20275 (4185858) 4185858..4189637 - 3780 WP_000060891.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T213082 WP_000239579.1 NZ_CP081698:c4185057-4184707 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T213082 NZ_CP107452:1468265-1468483 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT213082 WP_001223208.1 NZ_CP081698:c4185302-4185051 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT213082 NZ_CP107452:1467864-1468238 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References