Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3501721..3502339 Replicon chromosome
Accession NZ_CP081698
Organism Escherichia coli strain CT02

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J6N91_RS16905 Protein ID WP_001291435.1
Coordinates 3502121..3502339 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J6N91_RS16900 Protein ID WP_000344800.1
Coordinates 3501721..3502095 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6N91_RS16890 (3496810) 3496810..3498003 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J6N91_RS16895 (3498026) 3498026..3501175 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J6N91_RS16900 (3501721) 3501721..3502095 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J6N91_RS16905 (3502121) 3502121..3502339 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J6N91_RS16910 (3502511) 3502511..3503062 + 552 WP_000102564.1 maltose O-acetyltransferase -
J6N91_RS16915 (3503178) 3503178..3503648 + 471 WP_000136192.1 YlaC family protein -
J6N91_RS16920 (3503812) 3503812..3505362 + 1551 WP_001408474.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J6N91_RS16925 (3505404) 3505404..3505757 - 354 WP_223602053.1 DUF1428 family protein -
J6N91_RS16935 (3506136) 3506136..3506447 + 312 WP_000409911.1 MGMT family protein -
J6N91_RS16940 (3506478) 3506478..3507050 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213080 WP_001291435.1 NZ_CP081698:3502121-3502339 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T213080 NZ_CP081698:3502121-3502339 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT213080 WP_000344800.1 NZ_CP081698:3501721-3502095 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT213080 NZ_CP081698:3501721-3502095 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References