Detailed information of TA system TA213079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2530918..2531556
• Replicon: chromosome
• Accession: NZ_CP081698
• Organism: Escherichia coli strain CT02

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J6N91_RS12265
• Protein ID: WP_000813794.1
• Coordinates: 2531380..2531556 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J6N91_RS12260
• Protein ID: WP_001270286.1
• Coordinates: 2530918..2531334 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6N91_RS12240 (2526070)	2526070..2527011	-	942	WP_001251313.1	ABC transporter permease	-
J6N91_RS12245 (2527012)	2527012..2528025	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
J6N91_RS12250 (2528043)	2528043..2529188	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
J6N91_RS12255 (2529433)	2529433..2530839	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
J6N91_RS12260 (2530918)	2530918..2531334	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J6N91_RS12265 (2531380)	2531380..2531556	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J6N91_RS12270 (2531778)	2531778..2532008	+	231	WP_000494244.1	YncJ family protein	-
J6N91_RS12275 (2532100)	2532100..2534061	-	1962	WP_001488038.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J6N91_RS12280 (2534134)	2534134..2534670	-	537	WP_000429169.1	DNA-binding transcriptional regulator SutR	-
J6N91_RS12285 (2534762)	2534762..2535937	+	1176	WP_001236305.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2535977..2537125	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T213079 WP_000813794.1 NZ_CP081698:c2531556-2531380 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T213079 NZ_CP107450:c6004-5690 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT213079 WP_001270286.1 NZ_CP081698:c2531334-2530918 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT213079 NZ_CP107450:c6270-5992 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• PDB: 6HPB