Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 293718..294349 Replicon chromosome
Accession NZ_CP081698
Organism Escherichia coli strain CT02

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag J6N91_RS01310 Protein ID WP_001259388.1
Coordinates 293718..293993 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J6N91_RS01315 Protein ID WP_000593555.1
Coordinates 293990..294349 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6N91_RS01295 (288722) 288722..289789 + 1068 WP_000361470.1 HlyD family secretion protein -
J6N91_RS01300 (289786) 289786..292521 + 2736 WP_000149120.1 ribosome-associated ATPase/putative transporter RbbA -
J6N91_RS01305 (292521) 292521..293645 + 1125 WP_001216257.1 ABC transporter permease -
J6N91_RS01310 (293718) 293718..293993 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
J6N91_RS01315 (293990) 293990..294349 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J6N91_RS01320 (294469) 294469..294870 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J6N91_RS01325 (294876) 294876..295682 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J6N91_RS01330 (295679) 295679..296443 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J6N91_RS01335 (296443) 296443..297276 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J6N91_RS01340 (297273) 297273..298217 - 945 WP_000947062.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 275996..294349 18353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T213064 WP_001259388.1 NZ_CP081698:293718-293993 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T213064 NZ_CP107447:328021-328389 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT213064 WP_000593555.1 NZ_CP081698:293990-294349 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT213064 NZ_CP107447:327803-328024 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References