Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 39723..40526 Replicon plasmid unnamed2
Accession NZ_CP081692
Organism Escherichia coli strain 0H25

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag J6O25_RS26330 Protein ID WP_048213819.1
Coordinates 39996..40526 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag J6O25_RS26325 Protein ID WP_048213818.1
Coordinates 39723..39992 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O25_RS26295 35336..35476 - 141 Protein_29 arsenical pump-driving ATPase -
J6O25_RS26300 (J6O25_26165) 35596..36168 - 573 WP_001556711.1 recombinase family protein -
J6O25_RS26305 (J6O25_26170) 36332..36472 + 141 WP_160185462.1 hypothetical protein -
J6O25_RS26310 (J6O25_26175) 36484..37188 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O25_RS26315 (J6O25_26180) 37240..37596 + 357 Protein_33 site-specific integrase -
J6O25_RS26320 (J6O25_26185) 37919..38908 - 990 WP_191221256.1 RepB family plasmid replication initiator protein -
J6O25_RS26325 (J6O25_26190) 39723..39992 + 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
J6O25_RS26330 (J6O25_26195) 39996..40526 + 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
J6O25_RS26335 (J6O25_26200) 40611..41615 - 1005 WP_048213820.1 hypothetical protein -
J6O25_RS26340 (J6O25_26205) 41620..41865 - 246 WP_048213821.1 hypothetical protein -
J6O25_RS26345 (J6O25_26210) 42861..43247 + 387 WP_059593784.1 hypothetical protein -
J6O25_RS26350 (J6O25_26215) 43259..43792 + 534 WP_065212827.1 hypothetical protein -
J6O25_RS26355 (J6O25_26220) 43797..44576 + 780 WP_050316692.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..86367 86367


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T213062 WP_048213819.1 NZ_CP081692:39996-40526 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T213062 NZ_CP107445:c6004-5690 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT213062 WP_048213818.1 NZ_CP081692:39723-39992 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT213062 NZ_CP107445:c6270-5992 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References