Detailed information of TA system TA213061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 279654..280179
• Replicon: plasmid unnamed1
• Accession: NZ_CP081691
• Organism: Escherichia coli strain 0H25

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O25_RS26035
• Protein ID: WP_061301055.1
• Coordinates: 279874..280179 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: J6O25_RS26030
• Protein ID: WP_061301054.1
• Coordinates: 279654..279872 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O25_RS26000 (J6O25_25865)	275732..276481	+	750	WP_061301051.1	MobC family replication-relaxation protein	-
J6O25_RS26005 (J6O25_25870)	276519..276992	+	474	WP_011998898.1	thermonuclease family protein	-
J6O25_RS26010 (J6O25_25875)	277049..277393	+	345	WP_041460758.1	hypothetical protein	-
J6O25_RS26015 (J6O25_25880)	277432..277665	-	234	WP_011998900.1	hypothetical protein	-
J6O25_RS26020 (J6O25_25885)	277706..278353	-	648	WP_012815856.1	ParA family protein	-
J6O25_RS26025 (J6O25_25890)	278472..279254	-	783	WP_061301053.1	SprT-like domain-containing protein	-
J6O25_RS26030 (J6O25_25895)	279654..279872	+	219	WP_061301054.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O25_RS26035 (J6O25_25900)	279874..280179	+	306	WP_061301055.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O25_RS26040 (J6O25_25905)	280197..280751	+	555	WP_061301056.1	DUF5710 domain-containing protein	-
J6O25_RS26045 (J6O25_25910)	281030..282712	-	1683	WP_103216115.1	methyl-accepting chemotaxis protein	-
J6O25_RS26050 (J6O25_25915)	283268..283972	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O25_RS26055 (J6O25_25920)	283978..284742	+	765	WP_223584521.1	TnsA endonuclease N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..303843	303843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11409.04 Da
• Isoelectric Point: 4.5679

>T213061 WP_061301055.1 NZ_CP081691:279874-280179 [Escherichia coli]
MQFIIYEYKRVSHYKMFVDVQSDIVDTPGRRMVIPLIESHHLSEIVNSSLFPVVRINDEDYRVMTTELSSVSAAVTGEAI
ADVSSDADAIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213061 NZ_CP107443:c91487-91071 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8129.10 Da
• Isoelectric Point: 7.2032

>AT213061 WP_061301054.1 NZ_CP081691:279654-279872 [Escherichia coli]
MKHRVSVTVDRENYQVLSSAGINISGLVNEVIGKEARKIKAEQWKADNRAGMEEVANFIAQHGSFAEENRNW

Nucleotide

• Length: 219 bp

>AT213061 NZ_CP107443:c91714-91484 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA