Detailed information of TA system TA213060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 31145..31664
• Replicon: plasmid unnamed1
• Accession: NZ_CP081691
• Organism: Escherichia coli strain 0H25

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A837F4A9
• Locus tag: J6O25_RS24645
• Protein ID: WP_000277490.1
• Coordinates: 31145..31429 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6P1V8C3
• Locus tag: J6O25_RS24650
• Protein ID: WP_000124732.1
• Coordinates: 31422..31664 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O25_RS27365	26731..26853	-	123	WP_257606738.1	hypothetical protein	-
J6O25_RS24595 (J6O25_24475)	26897..27247	-	351	WP_000493399.1	hypothetical protein	-
J6O25_RS24600 (J6O25_24480)	27391..27852	-	462	WP_223584524.1	hypothetical protein	-
J6O25_RS24605 (J6O25_24485)	27809..28039	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	-
J6O25_RS24610 (J6O25_24490)	28036..28452	+	417	WP_088765932.1	type II toxin-antitoxin system VapC family toxin	-
J6O25_RS24615 (J6O25_24495)	28550..28690	-	141	WP_045286941.1	helix-turn-helix domain-containing protein	-
J6O25_RS24620 (J6O25_24500)	28789..29493	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O25_RS24625 (J6O25_24505)	29556..30077	+	522	Protein_40	glucose-1-phosphate adenylyltransferase	-
J6O25_RS24630 (J6O25_24510)	30077..30376	+	300	Protein_41	glycogen/starch synthase	-
J6O25_RS24635	30377..30595	+	219	Protein_42	IS110 family transposase	-
J6O25_RS24640 (J6O25_24515)	30915..31007	+	93	Protein_43	plasmid partition protein ParG	-
J6O25_RS24645 (J6O25_24520)	31145..31429	-	285	WP_000277490.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6O25_RS24650 (J6O25_24525)	31422..31664	-	243	WP_000124732.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
J6O25_RS24655 (J6O25_24530)	32237..33001	-	765	WP_000076190.1	plasmid replication initiator RepA	-
J6O25_RS24660 (J6O25_24535)	33067..35001	-	1935	Protein_47	Tn3 family transposase	-
J6O25_RS24665 (J6O25_24540)	35063..35767	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O25_RS24670 (J6O25_24545)	35829..35939	-	111	Protein_49	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..303843	303843
	-	inside	IScluster/Tn	-	-	17464..34920	17456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-88)

Antitoxin

• Antitoxin ParD (1-64)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11005.52 Da
• Isoelectric Point: 6.2103

>T213060 WP_000277490.1 NZ_CP081691:c31429-31145 [Escherichia coli]
VYKLTERAADDFAGIYDYTLLKFGEAQADHYTDALEAFFETLAGMPDMGRDYHTVPGVMRIEFQRHAIFYTVRDTDILIA
RILHQQMNHKRHLL

Nucleotide

• Length: 285 bp

>T213060 NZ_CP107443:c78398-78249 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8875.01 Da
• Isoelectric Point: 7.0130

>AT213060 WP_000124732.1 NZ_CP081691:c31664-31422 [Escherichia coli]
MARTTSVTIGEQLDSFISRLIDSGRYGSASEVMRSALRLLEQQETNDEVIRQAVIAGLESGESSLSLRDIAAQRKLRHRV

Nucleotide

• Length: 243 bp

>AT213060 NZ_CP107443:78443-78502 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A837F4A9

Antitoxin

• AlphaFold DB: A0A6P1V8C3