Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3700973..3701591 Replicon chromosome
Accession NZ_CP081690
Organism Escherichia coli strain 0H25

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J6O25_RS18090 Protein ID WP_001291435.1
Coordinates 3701373..3701591 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J6O25_RS18085 Protein ID WP_000344800.1
Coordinates 3700973..3701347 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O25_RS18075 (3696062) 3696062..3697255 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J6O25_RS18080 (3697278) 3697278..3700427 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J6O25_RS18085 (3700973) 3700973..3701347 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J6O25_RS18090 (3701373) 3701373..3701591 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J6O25_RS18095 (3701763) 3701763..3702314 + 552 WP_000102564.1 maltose O-acetyltransferase -
J6O25_RS18100 (3702430) 3702430..3702900 + 471 WP_000136192.1 YlaC family protein -
J6O25_RS18105 (3703064) 3703064..3704614 + 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J6O25_RS18110 (3704656) 3704656..3705009 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J6O25_RS18120 (3705388) 3705388..3705699 + 312 WP_000409911.1 MGMT family protein -
J6O25_RS18125 (3705730) 3705730..3706302 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213052 WP_001291435.1 NZ_CP081690:3701373-3701591 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T213052 NZ_CP107441:4190691-4190909 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT213052 WP_000344800.1 NZ_CP081690:3700973-3701347 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT213052 NZ_CP107441:4190290-4190664 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References