Detailed information of TA system TA021304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2941871..2942460
• Replicon: chromosome
• Accession: NC_007517
• Organism: Geobacter metallireducens GS-15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GMET_RS13010
• Protein ID: WP_081431817.1
• Coordinates: 2942206..2942460 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q39SG4
• Locus tag: GMET_RS13005
• Protein ID: WP_004514790.1
• Coordinates: 2941871..2942209 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMET_RS12980	2937494..2937835	+	342	WP_081431816.1	pentapeptide repeat-containing protein	-
GMET_RS12985	2937906..2938931	+	1026	WP_004514801.1	IS21-like element ISGme4 family transposase	-
GMET_RS12990	2938928..2939722	+	795	WP_004514800.1	IS21-like element ISGme4 family helper ATPase IstB	-
GMET_RS12995	2939767..2940255	+	489	WP_049756810.1	pentapeptide repeat-containing protein	-
GMET_RS13000	2940552..2941100	+	549	WP_004514792.1	hypothetical protein	-
GMET_RS18440	2941200..2941724	+	525	WP_004514791.1	DUF4410 domain-containing protein	-
GMET_RS13005	2941871..2942209	-	339	WP_004514790.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GMET_RS13010	2942206..2942460	-	255	WP_081431817.1	type II toxin-antitoxin system HicA family toxin	Toxin
GMET_RS13015	2942698..2943867	-	1170	WP_011365616.1	IS481-like element ISGme9 family transposase	-
GMET_RS13020	2944016..2944978	+	963	WP_004511867.1	SGNH/GDSL hydrolase family protein	-
GMET_RS13025	2945175..2945687	+	513	WP_011366073.1	hypothetical protein	-
GMET_RS13030	2945688..2946422	-	735	WP_004511865.1	NADH-quinone oxidoreductase subunit NuoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2937906..2943120	5214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9527.97 Da
• Isoelectric Point: 10.6470

>T21304 WP_081431817.1 NC_007517:c2942460-2942206 [Geobacter metallireducens GS-15]
MKRKNLKTLESIFAHPVPGNVQWPDILALFKELGAEITEREGSRVGVRLFGDRRVFHRPHPSPDTDKGAVASIRKWLEEN
GVKP

Nucleotide

• Length: 255 bp

>T21304 NC_007517:c2942460-2942206 [Geobacter metallireducens GS-15]
ATGAAAAGAAAAAATCTCAAAACACTGGAATCGATCTTTGCTCACCCGGTTCCCGGTAACGTTCAGTGGCCGGATATTCT
TGCACTTTTCAAAGAGCTTGGGGCTGAGATCACCGAGCGAGAAGGTTCCCGCGTCGGGGTTCGTCTATTTGGTGATCGAC
GAGTATTCCACCGTCCACATCCTTCGCCGGACACGGACAAAGGAGCAGTCGCTTCCATTCGGAAGTGGCTTGAAGAGAAT
GGAGTGAAACCATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12399.03 Da
• Isoelectric Point: 5.1460

>AT21304 WP_004514790.1 NC_007517:c2942209-2941871 [Geobacter metallireducens GS-15]
MNNMTINDYDAVISYDPEINMFRGEFVGINGGADFYASTVDDLRKEGEISLKAFLEMCAEEGVEPRKHFSGKFNLRVPPA
LHQRLAAQAAAHGKSINAWVVELLSECAVRGH

Nucleotide

• Length: 339 bp

>AT21304 NC_007517:c2942209-2941871 [Geobacter metallireducens GS-15]
ATGAACAATATGACCATAAACGACTATGACGCAGTCATTTCATATGACCCGGAAATCAACATGTTCCGGGGAGAGTTTGT
TGGAATCAATGGTGGTGCAGACTTCTACGCAAGTACTGTTGACGATCTTCGGAAAGAAGGCGAAATATCCCTCAAGGCTT
TTCTCGAAATGTGCGCCGAAGAAGGGGTTGAGCCGCGCAAACACTTCTCCGGAAAGTTCAACTTGCGGGTACCTCCCGCG
TTGCATCAGCGACTCGCTGCACAAGCTGCTGCTCACGGCAAGAGTATCAATGCCTGGGTTGTTGAACTCTTGTCAGAGTG
TGCAGTTCGCGGCCACTGA

Structures

Antitoxin

• AlphaFold DB: Q39SG4