Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 912462..913116 Replicon chromosome
Accession NZ_CP081690
Organism Escherichia coli strain 0H25

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag J6O25_RS04510 Protein ID WP_000244781.1
Coordinates 912709..913116 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J6O25_RS04505 Protein ID WP_000354046.1
Coordinates 912462..912728 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O25_RS04480 (907627) 907627..908369 + 743 Protein_880 SDR family oxidoreductase -
J6O25_RS04485 (908431) 908431..909864 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
J6O25_RS04490 (909909) 909909..910220 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
J6O25_RS04495 (910384) 910384..911043 + 660 WP_000250274.1 hemolysin III family protein -
J6O25_RS04500 (911239) 911239..912219 - 981 WP_000886082.1 tRNA-modifying protein YgfZ -
J6O25_RS04505 (912462) 912462..912728 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J6O25_RS04510 (912709) 912709..913116 + 408 WP_000244781.1 protein YgfX Toxin
J6O25_RS04515 (913156) 913156..913677 - 522 WP_001055868.1 flavodoxin FldB -
J6O25_RS04520 (913789) 913789..914685 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J6O25_RS04525 (914710) 914710..915420 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J6O25_RS04530 (915426) 915426..917159 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T213037 WP_000244781.1 NZ_CP081690:912709-913116 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T213037 NZ_CP107436:c4910521-4910237 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT213037 WP_000354046.1 NZ_CP081690:912462-912728 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT213037 NZ_CP107436:c4910753-4910511 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References