Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 57726..58529 Replicon plasmid unnamed2
Accession NZ_CP081686
Organism Escherichia coli strain 0H23

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag J6O16_RS26440 Protein ID WP_048213819.1
Coordinates 57999..58529 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag J6O16_RS26435 Protein ID WP_048213818.1
Coordinates 57726..57995 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O16_RS26405 53339..53479 - 141 Protein_53 arsenical pump-driving ATPase -
J6O16_RS26410 (J6O16_26320) 53599..54171 - 573 WP_001556711.1 recombinase family protein -
J6O16_RS26415 (J6O16_26325) 54335..54475 + 141 WP_160185462.1 hypothetical protein -
J6O16_RS26420 (J6O16_26330) 54487..55191 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O16_RS26425 (J6O16_26335) 55243..55599 + 357 Protein_57 site-specific integrase -
J6O16_RS26430 (J6O16_26340) 55922..56911 - 990 WP_191221256.1 RepB family plasmid replication initiator protein -
J6O16_RS26435 (J6O16_26345) 57726..57995 + 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
J6O16_RS26440 (J6O16_26350) 57999..58529 + 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
J6O16_RS26445 (J6O16_26355) 58614..59618 - 1005 WP_048213820.1 hypothetical protein -
J6O16_RS26450 (J6O16_26360) 59623..59868 - 246 WP_048213821.1 hypothetical protein -
J6O16_RS26455 (J6O16_26365) 60864..61250 + 387 WP_059593784.1 hypothetical protein -
J6O16_RS26460 (J6O16_26370) 61262..61795 + 534 WP_065212827.1 hypothetical protein -
J6O16_RS26465 (J6O16_26375) 61800..62579 + 780 WP_050316692.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..86361 86361


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T213032 WP_048213819.1 NZ_CP081686:57999-58529 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T213032 NZ_CP107436:877170-877577 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT213032 WP_048213818.1 NZ_CP081686:57726-57995 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT213032 NZ_CP107436:876923-877189 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References