Detailed information of TA system TA213031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 273397..273922
• Replicon: plasmid unnamed1
• Accession: NZ_CP081685
• Organism: Escherichia coli strain 0H23

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O16_RS25915
• Protein ID: WP_061301055.1
• Coordinates: 273397..273702 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: J6O16_RS25920
• Protein ID: WP_061301054.1
• Coordinates: 273704..273922 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O16_RS25895 (J6O16_25805)	268834..269598	-	765	WP_223584521.1	TnsA endonuclease N-terminal domain-containing protein	-
J6O16_RS25900 (J6O16_25810)	269604..270308	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O16_RS25905 (J6O16_25815)	270864..272546	+	1683	WP_103216115.1	methyl-accepting chemotaxis protein	-
J6O16_RS25910 (J6O16_25820)	272825..273379	-	555	WP_061301056.1	DUF5710 domain-containing protein	-
J6O16_RS25915 (J6O16_25825)	273397..273702	-	306	WP_061301055.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O16_RS25920 (J6O16_25830)	273704..273922	-	219	WP_061301054.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O16_RS25925 (J6O16_25835)	274322..275104	+	783	WP_061301053.1	SprT-like domain-containing protein	-
J6O16_RS25930 (J6O16_25840)	275223..275870	+	648	WP_012815856.1	ParA family protein	-
J6O16_RS25935 (J6O16_25845)	275911..276144	+	234	WP_011998900.1	hypothetical protein	-
J6O16_RS25940 (J6O16_25850)	276183..276527	-	345	WP_041460758.1	hypothetical protein	-
J6O16_RS25945 (J6O16_25855)	276584..277057	-	474	WP_011998898.1	thermonuclease family protein	-
J6O16_RS25950 (J6O16_25860)	277095..277844	-	750	WP_061301051.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..303841	303841
	-	flank	IS/Tn	-	-	269604..270308	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11409.04 Da
• Isoelectric Point: 4.5679

>T213031 WP_061301055.1 NZ_CP081685:c273702-273397 [Escherichia coli]
MQFIIYEYKRVSHYKMFVDVQSDIVDTPGRRMVIPLIESHHLSEIVNSSLFPVVRINDEDYRVMTTELSSVSAAVTGEAI
ADVSSDADAIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213031 NZ_CP107436:353992-354339 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8129.10 Da
• Isoelectric Point: 7.2032

>AT213031 WP_061301054.1 NZ_CP081685:c273922-273704 [Escherichia coli]
MKHRVSVTVDRENYQVLSSAGINISGLVNEVIGKEARKIKAEQWKADNRAGMEEVANFIAQHGSFAEENRNW

Nucleotide

• Length: 219 bp

>AT213031 NZ_CP107436:354339-354638 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA