Detailed information of TA system TA021303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2875653..2876249
• Replicon: chromosome
• Accession: NC_007517
• Organism: Geobacter metallireducens GS-15

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q39SM0
• Locus tag: GMET_RS12730
• Protein ID: WP_011366069.1
• Coordinates: 2875653..2875943 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GMET_RS12735
• Protein ID: WP_004514518.1
• Coordinates: 2875953..2876249 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMET_RS12710	2870657..2872096	-	1440	WP_004514522.1	insulinase family protein	-
GMET_RS12715	2872115..2873599	-	1485	WP_004514521.1	insulinase family protein	-
GMET_RS12720	2873755..2874726	-	972	WP_004514520.1	tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB	-
GMET_RS12725	2874723..2875457	-	735	WP_011366068.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
GMET_RS12730	2875653..2875943	+	291	WP_011366069.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GMET_RS12735	2875953..2876249	+	297	WP_004514518.1	putative addiction module antidote protein	Antitoxin
GMET_RS18430	2876660..2878267	-	1608	WP_004514517.1	hypothetical protein	-
GMET_RS12740	2878484..2878972	-	489	Protein_2560	IS5/IS1182 family transposase	-
GMET_RS12745	2879045..2879839	-	795	WP_004514800.1	IS21-like element ISGme4 family helper ATPase IstB	-
GMET_RS12750	2879836..2880861	-	1026	WP_004514801.1	IS21-like element ISGme4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10725.34 Da
• Isoelectric Point: 10.2736

>T21303 WP_011366069.1 NC_007517:2875653-2875943 [Geobacter metallireducens GS-15]
MIEIRKTEHFANWLDNLRDIQAKARVLVRIERLASGNAGDVKPVGEGVSEMRINYGPGYRVYFIQRGSELIILLAGGDKS
SQSRDIKAAVRLAQNL

Nucleotide

• Length: 291 bp

>T21303 NC_007517:2875653-2875943 [Geobacter metallireducens GS-15]
ATGATCGAAATCCGCAAAACCGAACATTTTGCCAACTGGCTCGATAACTTACGTGATATTCAAGCGAAGGCTCGTGTCCT
TGTAAGGATTGAGCGTCTTGCTTCTGGAAACGCAGGCGACGTTAAGCCTGTGGGTGAAGGTGTTTCGGAAATGCGGATCA
ACTACGGTCCCGGTTATCGAGTTTATTTTATCCAACGTGGCAGCGAATTGATCATCCTTTTGGCTGGTGGCGACAAGAGC
AGCCAGTCTCGGGATATCAAGGCAGCTGTCCGGCTTGCTCAGAACCTATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10479.89 Da
• Isoelectric Point: 6.2258

>AT21303 WP_004514518.1 NC_007517:2875953-2876249 [Geobacter metallireducens GS-15]
MTKTATSRYDVAEHLRTPEEMAAYLEASFEEANGDAAFIAKALGDIARAKGMSQVARDAGLSRESLYKALSGERVPGFDT
ILKIVSALGLKLHAEAAH

Nucleotide

• Length: 297 bp

>AT21303 NC_007517:2875953-2876249 [Geobacter metallireducens GS-15]
ATGACCAAAACAGCTACCAGTCGTTATGATGTTGCAGAACATCTCCGTACTCCTGAGGAAATGGCGGCCTATTTAGAAGC
CTCCTTTGAAGAGGCCAATGGCGATGCCGCTTTCATTGCAAAGGCTCTTGGCGACATAGCACGCGCTAAAGGTATGTCTC
AAGTGGCGCGCGATGCTGGCCTTTCTCGCGAAAGTCTTTATAAGGCACTTTCAGGTGAGCGTGTTCCTGGTTTTGATACT
ATTTTAAAAATCGTTTCAGCACTGGGACTCAAACTTCACGCAGAGGCCGCTCACTAG

Structures

Toxin

• AlphaFold DB: Q39SM0