Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4149802..4150352 Replicon chromosome
Accession NZ_CP081684
Organism Escherichia coli strain 0H23

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag J6O16_RS20355 Protein ID WP_001160971.1
Coordinates 4149802..4150116 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J6O16_RS20360 Protein ID WP_000125566.1
Coordinates 4150119..4150352 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O16_RS20330 (4145477) 4145477..4146763 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J6O16_RS20335 (4146763) 4146763..4147752 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J6O16_RS20340 (4147749) 4147749..4148570 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J6O16_RS20345 (4148573) 4148573..4148950 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J6O16_RS20350 (4148957) 4148957..4149805 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J6O16_RS20355 (4149802) 4149802..4150116 - 315 WP_001160971.1 CcdB family protein Toxin
J6O16_RS20360 (4150119) 4150119..4150352 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J6O16_RS20365 (4150438) 4150438..4150917 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
J6O16_RS20370 (4151109) 4151109..4152971 - 1863 WP_000377188.1 glutathione-regulated potassium-efflux system protein KefC -
J6O16_RS20375 (4152964) 4152964..4153494 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J6O16_RS20380 (4153605) 4153605..4154936 - 1332 WP_001183198.1 MFS transporter -
J6O16_RS20385 (4154994) 4154994..4155281 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T213024 WP_001160971.1 NZ_CP081684:c4150116-4149802 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T213024 NZ_CP107433:c86054-85905 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT213024 WP_000125566.1 NZ_CP081684:c4150352-4150119 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT213024 NZ_CP107433:86099-86158 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References