Detailed information of TA system TA021301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 2843788..2844359
• Replicon: chromosome
• Accession: NC_007517
• Organism: Geobacter metallireducens GS-15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q39SQ0
• Locus tag: GMET_RS12580
• Protein ID: WP_004514645.1
• Coordinates: 2843788..2844042 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GMET_RS12585
• Protein ID: WP_011366065.1
• Coordinates: 2844030..2844359 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMET_RS12565	2839090..2840565	-	1476	WP_004514649.1	anthranilate synthase component I	-
GMET_RS12570	2840812..2842764	+	1953	WP_004514648.1	molecular chaperone HtpG	-
GMET_RS12575	2842903..2843172	+	270	WP_004514647.1	DUF4160 domain-containing protein	-
GMET_RS18415	2843184..2843441	+	258	WP_004514646.1	DUF2442 domain-containing protein	-
GMET_RS18420	2843469..2843594	+	126	WP_081431800.1	DUF3387 domain-containing protein	-
GMET_RS12580	2843788..2844042	+	255	WP_004514645.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
GMET_RS12585	2844030..2844359	+	330	WP_011366065.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
GMET_RS18620	2844507..2844674	+	168	WP_004514643.1	hypothetical protein	-
GMET_RS12590	2844932..2845459	+	528	WP_004514642.1	L,D-transpeptidase family protein	-
GMET_RS12595	2845490..2846440	-	951	WP_004514641.1	rhomboid family intramembrane serine protease	-
GMET_RS12600	2846500..2847669	-	1170	WP_004514806.1	IS4-like element ISGme2 family transposase	-
GMET_RS12605	2847872..2849263	+	1392	WP_004514778.1	MATE family efflux transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2846500..2847669	1169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (7-48)

Antitoxin

• mRNA interferase PemK-like (3-106)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9723.85 Da
• Isoelectric Point: 4.3535

>T21301 WP_004514645.1 NC_007517:2843788-2844042 [Geobacter metallireducens GS-15]
MRTNIIRIGNSQGIRIPKVLLEQSHLGAEVELEVEDEKIIIRSASRPRQGWGEKFQLMAESGDDRMLNGDAGEQTEWDKD
EWEW

Nucleotide

• Length: 255 bp

>T21301 NC_007517:2843788-2844042 [Geobacter metallireducens GS-15]
ATGAGGACCAACATTATTCGCATAGGCAATTCCCAAGGCATCCGAATCCCTAAGGTTCTCCTGGAACAGAGCCATCTCGG
AGCAGAGGTGGAGTTGGAGGTGGAGGATGAGAAAATCATCATCCGCTCCGCTTCCCGCCCTCGCCAGGGGTGGGGGGAGA
AATTCCAGCTCATGGCTGAGAGCGGCGATGACAGAATGCTTAACGGGGATGCGGGCGAGCAGACCGAATGGGACAAGGAT
GAATGGGAATGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12446.62 Da
• Isoelectric Point: 9.5798

>AT21301 WP_011366065.1 NC_007517:2844030-2844359 [Geobacter metallireducens GS-15]
MGMVVKRFDVYLVNLDPTVGREIRKARPCLIISPDEMNRYIGTVIVAPMTTKGRDYPTRVQCSFKGKEGQVVLDQIRTVD
KSRLFQKLGRIDKETQEEVLSILAEMFAP

Nucleotide

• Length: 330 bp

>AT21301 NC_007517:2844030-2844359 [Geobacter metallireducens GS-15]
ATGGGAATGGTAGTCAAACGCTTCGACGTCTATCTGGTCAACCTGGACCCCACCGTCGGCAGGGAGATTCGCAAGGCAAG
GCCATGTCTCATTATCTCACCCGATGAAATGAACCGGTACATCGGCACCGTTATCGTTGCCCCCATGACCACCAAAGGGA
GGGATTATCCCACGCGGGTGCAGTGCTCCTTCAAGGGCAAGGAGGGACAGGTTGTCCTTGACCAGATCAGAACCGTGGAC
AAGTCCCGTCTTTTCCAGAAGCTCGGAAGAATCGACAAAGAAACGCAGGAAGAGGTGTTGTCCATTCTTGCGGAGATGTT
TGCGCCGTAA

Structures

Toxin

• AlphaFold DB: Q39SQ0