Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 37580..38383 Replicon plasmid unnamed2
Accession NZ_CP081679
Organism Escherichia coli strain 0H15

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag J6O45_RS26395 Protein ID WP_048213819.1
Coordinates 37580..38110 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag J6O45_RS26400 Protein ID WP_048213818.1
Coordinates 38114..38383 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O45_RS26370 (J6O45_26240) 33530..34309 - 780 WP_050316692.1 site-specific integrase -
J6O45_RS26375 (J6O45_26245) 34314..34847 - 534 WP_065212827.1 hypothetical protein -
J6O45_RS26380 (J6O45_26250) 34859..35245 - 387 WP_059593784.1 hypothetical protein -
J6O45_RS26385 (J6O45_26255) 36241..36486 + 246 WP_048213821.1 hypothetical protein -
J6O45_RS26390 (J6O45_26260) 36491..37495 + 1005 WP_048213820.1 hypothetical protein -
J6O45_RS26395 (J6O45_26265) 37580..38110 - 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
J6O45_RS26400 (J6O45_26270) 38114..38383 - 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
J6O45_RS26405 (J6O45_26275) 39198..40187 + 990 WP_191221256.1 RepB family plasmid replication initiator protein -
J6O45_RS26410 (J6O45_26280) 40510..40866 - 357 Protein_53 site-specific integrase -
J6O45_RS26415 (J6O45_26285) 40918..41622 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O45_RS26420 (J6O45_26290) 41634..41774 - 141 WP_160185462.1 hypothetical protein -
J6O45_RS26425 (J6O45_26295) 41938..42510 + 573 WP_001556711.1 recombinase family protein -
J6O45_RS26430 42630..42770 + 141 Protein_57 arsenical pump-driving ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..86367 86367


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T213002 WP_048213819.1 NZ_CP081679:c38110-37580 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T213002 NZ_CP107426:c4003157-4002939 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT213002 WP_048213818.1 NZ_CP081679:c38383-38114 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT213002 NZ_CP107426:c4003558-4003184 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References