Detailed information of TA system TA213001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 254984..255509
• Replicon: plasmid unnamed1
• Accession: NZ_CP081678
• Organism: Escherichia coli strain 0H15

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O45_RS25880
• Protein ID: WP_061301055.1
• Coordinates: 255204..255509 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: J6O45_RS25875
• Protein ID: WP_061301054.1
• Coordinates: 254984..255202 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O45_RS25845 (J6O45_25715)	251062..251811	+	750	WP_061301051.1	MobC family replication-relaxation protein	-
J6O45_RS25850 (J6O45_25720)	251849..252322	+	474	WP_011998898.1	thermonuclease family protein	-
J6O45_RS25855 (J6O45_25725)	252379..252723	+	345	WP_041460758.1	hypothetical protein	-
J6O45_RS25860 (J6O45_25730)	252762..252995	-	234	WP_011998900.1	hypothetical protein	-
J6O45_RS25865 (J6O45_25735)	253036..253683	-	648	WP_012815856.1	ParA family protein	-
J6O45_RS25870 (J6O45_25740)	253802..254584	-	783	WP_061301053.1	SprT-like domain-containing protein	-
J6O45_RS25875 (J6O45_25745)	254984..255202	+	219	WP_061301054.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O45_RS25880 (J6O45_25750)	255204..255509	+	306	WP_061301055.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O45_RS25885 (J6O45_25755)	255527..256081	+	555	WP_061301056.1	DUF5710 domain-containing protein	-
J6O45_RS25890 (J6O45_25760)	256360..258042	-	1683	WP_103216115.1	methyl-accepting chemotaxis protein	-
J6O45_RS25895 (J6O45_25765)	258598..259302	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O45_RS25900 (J6O45_25770)	259308..260072	+	765	WP_223584521.1	TnsA endonuclease N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..303841	303841
	-	flank	IS/Tn	-	-	258598..259302	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11409.04 Da
• Isoelectric Point: 4.5679

>T213001 WP_061301055.1 NZ_CP081678:255204-255509 [Escherichia coli]
MQFIIYEYKRVSHYKMFVDVQSDIVDTPGRRMVIPLIESHHLSEIVNSSLFPVVRINDEDYRVMTTELSSVSAAVTGEAI
ADVSSDADAIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213001 NZ_CP107426:3389135-3389668 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8129.10 Da
• Isoelectric Point: 7.2032

>AT213001 WP_061301054.1 NZ_CP081678:254984-255202 [Escherichia coli]
MKHRVSVTVDRENYQVLSSAGINISGLVNEVIGKEARKIKAEQWKADNRAGMEEVANFIAQHGSFAEENRNW

Nucleotide

• Length: 219 bp

>AT213001 NZ_CP107426:3388858-3389124 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA