Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3139..3782 Replicon plasmid unnamed1
Accession NZ_CP081678
Organism Escherichia coli strain 0H15

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag J6O45_RS24465 Protein ID WP_088765932.1
Coordinates 3366..3782 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J6O45_RS24460 Protein ID WP_001261282.1
Coordinates 3139..3369 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O45_RS24440 (J6O45_24315) 98..1270 + 1173 WP_042111630.1 IS21-like element IS21 family transposase -
J6O45_RS24445 (J6O45_24320) 1270..2067 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
J6O45_RS27130 2061..2183 - 123 WP_257606738.1 hypothetical protein -
J6O45_RS24450 (J6O45_24325) 2227..2577 - 351 WP_000493399.1 hypothetical protein -
J6O45_RS24455 (J6O45_24330) 2721..3182 - 462 WP_223584524.1 hypothetical protein -
J6O45_RS24460 (J6O45_24335) 3139..3369 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O45_RS24465 (J6O45_24340) 3366..3782 + 417 WP_088765932.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O45_RS24470 (J6O45_24345) 3880..4020 - 141 WP_045286941.1 helix-turn-helix domain-containing protein -
J6O45_RS24475 (J6O45_24350) 4119..4823 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O45_RS24480 (J6O45_24355) 4886..5407 + 522 Protein_10 glucose-1-phosphate adenylyltransferase -
J6O45_RS24485 (J6O45_24360) 5407..5706 + 300 Protein_11 glycogen/starch synthase -
J6O45_RS24490 5707..5925 + 219 Protein_12 IS110 family transposase -
J6O45_RS24495 (J6O45_24365) 6245..6337 + 93 Protein_13 plasmid partition protein ParG -
J6O45_RS24500 (J6O45_24370) 6475..6759 - 285 WP_000277490.1 type II toxin-antitoxin system RelE/ParE family toxin -
J6O45_RS24505 (J6O45_24375) 6752..6994 - 243 WP_000124732.1 type II toxin-antitoxin system ParD family antitoxin -
J6O45_RS24510 (J6O45_24380) 7567..8379 - 813 WP_257606735.1 plasmid replication initiator RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..303841 303841
- inside IScluster/Tn - - 98..11097 10999


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15164.56 Da        Isoelectric Point: 6.4581

>T212999 WP_088765932.1 NZ_CP081678:3366-3782 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T212999 NZ_CP107426:3129986-3130903 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT212999 WP_001261282.1 NZ_CP081678:3139-3369 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT212999 NZ_CP107426:3129222-3129980 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References