Detailed information of TA system TA212997

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4476011..4476804
• Replicon: chromosome
• Accession: NZ_CP081677
• Organism: Escherichia coli strain 0H15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: J6O45_RS22000
• Protein ID: WP_000854726.1
• Coordinates: 4476427..4476804 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: J6O45_RS21995
• Protein ID: WP_001548930.1
• Coordinates: 4476011..4476337 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O45_RS21950 (4471498)	4471498..4472142	-	645	WP_001547746.1	TetR/AcrR family transcriptional regulator	-
J6O45_RS21960 (4473229)	4473229..4473465	+	237	WP_001144031.1	protein YpjK	-
J6O45_RS21965 (4473465)	4473465..4473908	+	444	WP_001547764.1	lipoprotein YafY	-
J6O45_RS21970 (4473931)	4473931..4474398	+	468	WP_001547765.1	protein YkfB	-
J6O45_RS21975 (4474475)	4474475..4474714	+	240	WP_000194654.1	DUF905 family protein	-
J6O45_RS21980 (4474812)	4474812..4475270	+	459	WP_001548150.1	antirestriction protein	-
J6O45_RS21985 (4475286)	4475286..4475762	+	477	WP_000811693.1	RadC family protein	-
J6O45_RS21990 (4475771)	4475771..4475992	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O45_RS21995 (4476011)	4476011..4476337	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O45_RS22000 (4476427)	4476427..4476804	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
J6O45_RS22005 (4476801)	4476801..4477289	+	489	WP_001547769.1	DUF5983 family protein	-
J6O45_RS22010 (4477301)	4477301..4477498	+	198	WP_000839269.1	DUF957 domain-containing protein	-
J6O45_RS22015 (4477583)	4477583..4478425	+	843	WP_001547770.1	DUF4942 domain-containing protein	-
J6O45_RS22020 (4479174)	4479174..4480712	+	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4440875..4488525	47650
	-	flank	IS/Tn	-	-	4472417..4472794	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T212997 WP_000854726.1 NZ_CP081677:4476427-4476804 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T212997 NZ_CP107426:c2400701-2400333 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT212997 WP_001548930.1 NZ_CP081677:4476011-4476337 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT212997 NZ_CP107426:c2400919-2400698 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7