Detailed information of TA system TA212993

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3901120..3901799
• Replicon: chromosome
• Accession: NZ_CP081677
• Organism: Escherichia coli strain 0H15

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O45_RS19100
• Protein ID: WP_000854680.1
• Coordinates: 3901458..3901799 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O45_RS19095
• Protein ID: WP_000070396.1
• Coordinates: 3901120..3901437 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O45_RS19050 (3896558)	3896558..3897379	+	822	WP_000197388.1	DUF932 domain-containing protein	-
J6O45_RS19055 (3897596)	3897596..3898297	+	702	WP_000189411.1	WYL domain-containing protein	-
J6O45_RS19060 (3898338)	3898338..3898574	+	237	WP_001144031.1	protein YpjK	-
J6O45_RS19065 (3898574)	3898574..3899017	+	444	WP_001547764.1	lipoprotein YafY	-
J6O45_RS19070 (3899040)	3899040..3899507	+	468	WP_001547765.1	protein YkfB	-
J6O45_RS19075 (3899584)	3899584..3899823	+	240	WP_000194654.1	DUF905 family protein	-
J6O45_RS19080 (3899921)	3899921..3900379	+	459	WP_001548150.1	antirestriction protein	-
J6O45_RS19085 (3900395)	3900395..3900871	+	477	WP_000811693.1	RadC family protein	-
J6O45_RS19090 (3900880)	3900880..3901101	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O45_RS19095 (3901120)	3901120..3901437	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O45_RS19100 (3901458)	3901458..3901799	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O45_RS19105 (3902620)	3902620..3904086	-	1467	WP_000723924.1	hypothetical protein	-
J6O45_RS19110 (3904402)	3904402..3904821	-	420	WP_001333214.1	hypothetical protein	-
J6O45_RS19115 (3905035)	3905035..3905121	-	87	Protein_3750	capsid protein	-
J6O45_RS19120 (3905326)	3905326..3906099	+	774	WP_000355484.1	hypothetical protein	-
J6O45_RS19125 (3906160)	3906160..3906714	-	555	WP_000905043.1	site-specific DNA recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3876175..3914537	38362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T212993 WP_000854680.1 NZ_CP081677:3901458-3901799 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T212993 NZ_CP107425:2792-3058 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT212993 WP_000070396.1 NZ_CP081677:3901120-3901437 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT212993 NZ_CP107425:2584-2808 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7