Detailed information of TA system TA021299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 4781..5261
• Replicon: plasmid unnamed
• Accession: NC_007515
• Organism: Geobacter metallireducens GS-15

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: Q39PQ0
• Locus tag: GMET_RS17990
• Protein ID: WP_004514725.1
• Coordinates: 4781..5053 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: Q39PP9
• Locus tag: GMET_RS17995
• Protein ID: WP_004514724.1
• Coordinates: 5037..5261 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMET_RS17960	963..2063	-	1101	WP_004514711.1	hypothetical protein	-
GMET_RS17965	2067..2378	-	312	WP_148206096.1	hypothetical protein	-
GMET_RS17970	2393..3562	-	1170	WP_011363043.1	IS4-like element ISGme2 family transposase	-
GMET_RS17975	3862..4071	-	210	WP_004514728.1	hypothetical protein	-
GMET_RS17980	4068..4409	-	342	WP_004514727.1	hypothetical protein	-
GMET_RS17985	4575..4784	-	210	WP_004514726.1	hypothetical protein	-
GMET_RS17990	4781..5053	-	273	WP_004514725.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GMET_RS17995	5037..5261	-	225	WP_004514724.1	ribbon-helix-helix protein, CopG family	Antitoxin
GMET_RS18000	5321..5809	-	489	WP_004514723.1	hypothetical protein	-
GMET_RS18005	6011..6196	-	186	WP_004514722.1	hypothetical protein	-
GMET_RS18010	6237..6956	-	720	WP_004514721.1	P-type conjugative transfer protein TrbJ	-
GMET_RS18015	7043..8494	-	1452	WP_011363044.1	P-type conjugative transfer protein TrbL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..13762	13762
	-	flank	IS/Tn	-	-	2393..3562	1169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (3-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10607.32 Da
• Isoelectric Point: 10.2148

>T21299 WP_004514725.1 NC_007515:c5053-4781 [Geobacter metallireducens GS-15]
MVWKVEIDPAARRELKKLDPQISGRVLKFLFERVARLDDPRSIGEALKGSRFGDFWKYRVGDYRIITSIEDEALVILVVR
VGNRREVYER

Nucleotide

• Length: 273 bp

>T21299 NC_007515:c5053-4781 [Geobacter metallireducens GS-15]
ATGGTCTGGAAAGTTGAAATAGACCCCGCCGCCCGGCGGGAATTAAAGAAGCTGGACCCTCAAATAAGCGGGAGGGTCTT
GAAATTCCTCTTTGAGCGTGTCGCACGCCTTGACGATCCCCGCAGTATCGGGGAGGCCCTGAAGGGTTCCAGATTCGGAG
ACTTTTGGAAATACCGCGTAGGTGACTATAGGATCATCACCAGCATAGAAGACGAGGCGTTAGTAATTCTCGTTGTGAGG
GTAGGCAACCGCCGGGAGGTCTACGAAAGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8607.84 Da
• Isoelectric Point: 4.4410

>AT21299 WP_004514724.1 NC_007515:c5261-5037 [Geobacter metallireducens GS-15]
MLAIRLPEEIETRLDSLAKATGRSKSYYVREALVEYLDDLEDIYLAEKRLEDIRAGRTKTIPLEEVMKEYGLES

Nucleotide

• Length: 225 bp

>AT21299 NC_007515:c5261-5037 [Geobacter metallireducens GS-15]
ATGTTAGCAATCAGACTGCCCGAGGAAATCGAAACCCGCCTTGACTCCCTCGCCAAGGCCACCGGCCGGAGCAAAAGCTA
TTACGTCCGGGAGGCCCTTGTTGAATATCTTGACGACCTGGAAGACATTTACTTGGCCGAGAAGCGCCTTGAAGACATTA
GGGCCGGACGAACCAAAACCATACCCCTTGAAGAAGTGATGAAAGAATATGGTCTGGAAAGTTGA

Structures

Toxin

• AlphaFold DB: Q39PQ0

Antitoxin

• AlphaFold DB: Q39PP9