Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 322301..322932 Replicon chromosome
Accession NZ_CP081677
Organism Escherichia coli strain 0H15

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag J6O45_RS01495 Protein ID WP_001259385.1
Coordinates 322301..322576 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J6O45_RS01500 Protein ID WP_000593555.1
Coordinates 322573..322932 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O45_RS01480 (317305) 317305..318372 + 1068 WP_000361470.1 HlyD family secretion protein -
J6O45_RS01485 (318369) 318369..321104 + 2736 WP_000149123.1 ribosome-associated ATPase/putative transporter RbbA -
J6O45_RS01490 (321104) 321104..322228 + 1125 WP_001488398.1 ABC transporter permease -
J6O45_RS01495 (322301) 322301..322576 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
J6O45_RS01500 (322573) 322573..322932 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J6O45_RS01505 (323052) 323052..323453 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J6O45_RS01510 (323459) 323459..324265 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J6O45_RS01515 (324262) 324262..325026 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J6O45_RS01520 (325026) 325026..325859 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J6O45_RS01525 (325856) 325856..326800 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 299563..322932 23369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T212974 WP_001259385.1 NZ_CP081677:322301-322576 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T212974 NZ_CP107418:80803-80952 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT212974 WP_000593555.1 NZ_CP081677:322573-322932 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT212974 NZ_CP107418:c80758-80699 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References