Detailed information of TA system TA212972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 3405869..3406874
• Replicon: chromosome
• Accession: NZ_CP081673
• Organism: Mycolicibacterium farcinogenes strain BKK/CU-MFGFA-001

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: K6L26_RS16165
• Protein ID: WP_221850772.1
• Coordinates: 3405869..3406447 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A378T1G9
• Locus tag: K6L26_RS16170
• Protein ID: WP_019346253.1
• Coordinates: 3406485..3406874 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6L26_RS16140 (K6L26_16140)	3400949..3401788	+	840	WP_109789989.1	thioesterase family protein	-
K6L26_RS16145 (K6L26_16145)	3401742..3402347	-	606	WP_221850769.1	class I SAM-dependent methyltransferase	-
K6L26_RS16150 (K6L26_16150)	3402400..3403035	-	636	WP_036387324.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
K6L26_RS16155 (K6L26_16155)	3403126..3403557	+	432	WP_036387326.1	Fur family transcriptional regulator	-
K6L26_RS16160 (K6L26_16160)	3403593..3405848	+	2256	WP_221850770.1	catalase/peroxidase HPI	-
K6L26_RS16165 (K6L26_16165)	3405869..3406447	-	579	WP_221850772.1	zeta toxin family protein	Toxin
K6L26_RS16170 (K6L26_16170)	3406485..3406874	-	390	WP_019346253.1	hypothetical protein	Antitoxin
K6L26_RS16175 (K6L26_16175)	3406942..3407958	-	1017	WP_221850774.1	TIGR03617 family F420-dependent LLM class oxidoreductase	-
K6L26_RS16180 (K6L26_16180)	3408086..3409441	+	1356	WP_221850776.1	alpha/beta hydrolase-fold protein	-
K6L26_RS16185 (K6L26_16185)	3409840..3410250	+	411	WP_019346256.1	transglycosylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-140)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 20926.04 Da
• Isoelectric Point: 7.0515

>T212972 WP_221850772.1 NZ_CP081673:c3406447-3405869 [Mycolicibacterium farcinogenes]
VKRLDLVVGCNGAGKSTFVELTLSPLLPGSPFVNADEIAKRRWPEDPAPHSYEAALIAAETRAKLIELGESFIAETVFSH
PSKLDLIDDAHIAGYTVVLHCILIPEELAVQRVRHRVSAGGHDVPEAKIRERYQRLWDLVAVAAMRADAATFYDNSTVRG
PRIVAQIAGGFAIGSPTWPKWAPAVLISRWAG

Nucleotide

• Length: 579 bp

>T212972 NZ_CP107417:c5386257-5385874 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13745.15 Da
• Isoelectric Point: 5.2329

>AT212972 WP_019346253.1 NZ_CP081673:c3406874-3406485 [Mycolicibacterium farcinogenes]
MTETADRVTRFAADLIDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHSAARRKVESALAGELSLRELTAEEGTVFDAEI
AASIEESLARADYGKTLAGRGITTVALDDNGNIVQYRPDGSTAVLARTQ

Nucleotide

• Length: 390 bp

>AT212972 NZ_CP107417:c5386499-5386257 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A378T1G9