Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907(toxin) |
| Location | 62760..63331 | Replicon | plasmid pE006-TC-121 |
| Accession | NZ_CP081506 | ||
| Organism | Enterococcus faecalis strain E006xJH2-2-TC1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A2Z2CGM4 |
| Locus tag | K4752_RS14415 | Protein ID | WP_002362432.1 |
| Coordinates | 62990..63331 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | R3KHK9 |
| Locus tag | K4752_RS14410 | Protein ID | WP_002362431.1 |
| Coordinates | 62760..62990 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K4752_RS14370 (58704) | 58704..59003 | - | 300 | WP_010817802.1 | hypothetical protein | - |
| - (59112) | 59112..59203 | + | 92 | NuclAT_0 | - | - |
| - (59112) | 59112..59203 | + | 92 | NuclAT_0 | - | - |
| - (59112) | 59112..59203 | + | 92 | NuclAT_0 | - | - |
| - (59112) | 59112..59203 | + | 92 | NuclAT_0 | - | - |
| K4752_RS14375 (59243) | 59243..59353 | - | 111 | WP_002360667.1 | type I toxin-antitoxin system Fst family toxin | - |
| K4752_RS14380 (59848) | 59848..60165 | + | 318 | WP_002394802.1 | heavy metal-binding domain-containing protein | - |
| K4752_RS14385 (60201) | 60201..60869 | + | 669 | WP_002403283.1 | CPBP family intramembrane metalloprotease | - |
| K4752_RS14390 (60986) | 60986..61240 | + | 255 | WP_002394800.1 | hypothetical protein | - |
| K4752_RS14395 (61400) | 61400..61633 | - | 234 | WP_002394799.1 | hypothetical protein | - |
| K4752_RS14400 (61635) | 61635..61919 | - | 285 | WP_002394798.1 | hypothetical protein | - |
| K4752_RS14405 (61936) | 61936..62556 | - | 621 | WP_162500025.1 | recombinase family protein | - |
| K4752_RS14410 (62760) | 62760..62990 | + | 231 | WP_002362431.1 | hypothetical protein | Antitoxin |
| K4752_RS14415 (62990) | 62990..63331 | + | 342 | WP_002362432.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| K4752_RS14420 (63443) | 63443..64381 | + | 939 | WP_002362433.1 | hypothetical protein | - |
| K4752_RS14425 (64646) | 64646..65248 | + | 603 | WP_010711821.1 | Fic family protein | - |
| K4752_RS14430 (65497) | 65497..67335 | + | 1839 | WP_010711822.1 | P-loop NTPase fold protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | erm(B) / ant(6)-Ia / aac(6')-aph(2'') / dfrG / lnu(G) / erm(A) / optrA / poxtA / fexB | prgB/asc10 | 1..121520 | 121520 | |
| - | flank | IS/Tn | - | - | 68159..68845 | 686 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 13244.44 Da Isoelectric Point: 8.0113
>T212969 WP_002362432.1 NZ_CP081506:62990-63331 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
Download Length: 342 bp
>T212969 NZ_CP107417:c4803384-4802851 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8510.42 Da Isoelectric Point: 4.3855
>AT212969 WP_002362431.1 NZ_CP081506:62760-62990 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
Download Length: 231 bp
>AT212969 NZ_CP107417:c4803661-4803395 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z2CGM4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R3KHK9 |