Detailed information of TA system TA212944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2784703..2785274
• Replicon: chromosome
• Accession: NZ_CP081505
• Organism: Enterococcus faecalis strain E006xJH2-2-TC1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K4752_RS13565
• Protein ID: WP_002385138.1
• Coordinates: 2784703..2785044 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: K4752_RS13570
• Protein ID: WP_002354773.1
• Coordinates: 2785044..2785274 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4752_RS13560 (2780718)	2780718..2784332	-	3615	WP_010774058.1	DNA-directed RNA polymerase subunit beta	-
K4752_RS13565 (2784703)	2784703..2785044	-	342	WP_002385138.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K4752_RS13570 (2785044)	2785044..2785274	-	231	WP_002354773.1	hypothetical protein	Antitoxin
K4752_RS13575 (2785680)	2785680..2785895	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
K4752_RS13580 (2786034)	2786034..2787026	+	993	WP_002385136.1	biotin--[acetyl-CoA-carboxylase] ligase	-
K4752_RS13585 (2787290)	2787290..2787928	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
K4752_RS13590 (2788616)	2788616..2790232	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13128.35 Da
• Isoelectric Point: 9.3984

>T212944 WP_002385138.1 NZ_CP081505:c2785044-2784703 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAAICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T212944 NZ_CP107410:c4371-4057 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT212944 WP_002354773.1 NZ_CP081505:c2785274-2785044 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT212944 NZ_CP107410:c4637-4359 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812