Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 2482108..2482690 Replicon chromosome
Accession NZ_CP081505
Organism Enterococcus faecalis strain E006xJH2-2-TC1

Toxin (Protein)


Gene name zeta Uniprot ID A0A0M2AE74
Locus tag K4752_RS12185 Protein ID WP_002355414.1
Coordinates 2482108..2482416 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag K4752_RS12190 Protein ID WP_002326825.1
Coordinates 2482418..2482690 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K4752_RS12155 (2477895) 2477895..2478827 - 933 WP_002363040.1 tagatose-6-phosphate kinase -
K4752_RS12160 (2478838) 2478838..2479353 - 516 WP_002345825.1 galactose-6-phosphate isomerase subunit LacB -
K4752_RS12165 (2479370) 2479370..2479795 - 426 WP_002355418.1 galactose-6-phosphate isomerase subunit LacA -
K4752_RS12170 (2480283) 2480283..2481050 + 768 WP_002384821.1 DeoR/GlpR family DNA-binding transcription regulator -
K4752_RS12175 (2481055) 2481055..2481555 + 501 WP_002355415.1 HAD family hydrolase -
K4752_RS12180 (2481606) 2481606..2482028 + 423 WP_080005963.1 tyrosine-type recombinase/integrase -
K4752_RS12185 (2482108) 2482108..2482416 - 309 WP_002355414.1 zeta toxin family protein Toxin
K4752_RS12190 (2482418) 2482418..2482690 - 273 WP_002326825.1 antitoxin Antitoxin
K4752_RS12195 (2482707) 2482707..2482922 - 216 WP_002364919.1 peptide-binding protein -
K4752_RS12200 (2482993) 2482993..2483847 - 855 WP_002364921.1 ParA family protein -
K4752_RS12205 (2483955) 2483955..2485117 + 1163 WP_107164651.1 IS3 family transposase -
K4752_RS14860 (2485333) 2485333..2487011 - 1679 Protein_2385 DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element ClpL bsh 2354878..2495967 141089


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-99)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11525.91 Da        Isoelectric Point: 5.7251

>T212943 WP_002355414.1 NZ_CP081505:c2482416-2482108 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHATSYSNQLVKLN

Download         Length: 309 bp

>T212943 NZ_CP107409:156784-157200 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT212943 WP_002326825.1 NZ_CP081505:c2482690-2482418 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT212943 NZ_CP107409:156557-156787 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AE74

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References