Detailed information of TA system TA212935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-ParD
• Location: 223663..224227
• Replicon: chromosome
• Accession: NZ_CP081496
• Organism: Streptomyces sp. BHT-5-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K2224_RS01005
• Protein ID: WP_221909353.1
• Coordinates: 223663..224013 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K2224_RS01010
• Protein ID: WP_221904726.1
• Coordinates: 223997..224227 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2224_RS00995 (K2224_00995)	221295..222320	+	1026	WP_221904724.1	hypothetical protein	-
K2224_RS01000 (K2224_01000)	222536..223567	+	1032	WP_221904725.1	aspartate-semialdehyde dehydrogenase	-
K2224_RS01005 (K2224_01005)	223663..224013	-	351	WP_221909353.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K2224_RS01010 (K2224_01010)	223997..224227	-	231	WP_221904726.1	ribbon-helix-helix domain-containing protein	Antitoxin
K2224_RS01015 (K2224_01015)	224350..226923	+	2574	WP_221904727.1	aminopeptidase N	-
K2224_RS01020 (K2224_01020)	227192..228337	-	1146	WP_221904728.1	allantoicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12759.74 Da
• Isoelectric Point: 10.4500

>T212935 WP_221909353.1 NZ_CP081496:c224013-223663 [Streptomyces sp. BHT-5-2]
MMNRGDIYLVDLEPAVGSEANKQRPAVLVTNNAANRMARRAGRGVLAVVPVTSNVAHVRPFQVFLHSTECGLKVDSKAQC
EQIRALDFSRFGQQVGVVPPHTLKEIDRALRQQLAL

Nucleotide

• Length: 351 bp

>T212935 NZ_CP107408:2065159-2065261 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8607.45 Da
• Isoelectric Point: 3.9067

>AT212935 WP_221904726.1 NZ_CP081496:c224227-223997 [Streptomyces sp. BHT-5-2]
MKISVSLPQEDVDFVDEYAAKKAAESRSAVIHAAIQMLREVALEEEYLAAWDEWYASEDAELWDRTVGDGLSDESR

Nucleotide

• Length: 231 bp

>AT212935 NZ_CP107408:c2065268-2065123 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT