Detailed information of TA system TA212928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1286645..1287276
• Replicon: chromosome
• Accession: NZ_CP081490
• Organism: Pseudomonas protegens strain PS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K5F93_RS06115
• Protein ID: WP_221810962.1
• Coordinates: 1286878..1287276 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K5F93_RS06110
• Protein ID: WP_102860368.1
• Coordinates: 1286645..1286878 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5F93_RS06085 (K5F93_06085)	1282315..1283442	+	1128	WP_110652277.1	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	-
K5F93_RS06090 (K5F93_06090)	1283482..1283841	+	360	WP_221810959.1	amidohydrolase family protein	-
K5F93_RS06095 (K5F93_06095)	1283976..1284416	+	441	WP_221810960.1	DUF3592 domain-containing protein	-
K5F93_RS06100 (K5F93_06100)	1284421..1285005	+	585	WP_060840910.1	hypothetical protein	-
K5F93_RS06105 (K5F93_06105)	1285030..1286277	-	1248	WP_221810961.1	aspartate aminotransferase family protein	-
K5F93_RS06110 (K5F93_06110)	1286645..1286878	+	234	WP_102860368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K5F93_RS06115 (K5F93_06115)	1286878..1287276	+	399	WP_221810962.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K5F93_RS06120 (K5F93_06120)	1287299..1287412	-	114	WP_260627405.1	DUF2938 domain-containing protein	-
K5F93_RS06125 (K5F93_06125)	1287753..1289801	+	2049	WP_221810963.1	c-type cytochrome	-
K5F93_RS06130 (K5F93_06130)	1289938..1290507	+	570	WP_221810964.1	hypothetical protein	-
K5F93_RS06135 (K5F93_06135)	1290517..1290879	-	363	WP_221810965.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15063.42 Da
• Isoelectric Point: 7.9035

>T212928 WP_221810962.1 NZ_CP081490:1286878-1287276 [Pseudomonas protegens]
MLKYMLDTNICIFTIKNKPEQVREAFRRHQGRLCISTITLMELIYGAEKSAIPEKNLSRVESFVARLQVLDYDDAAAIHS
GQLHAELVRQGKQIGPYDQLIAGHARSLGLTLITNNLREFERVPGLRVEDWV

Nucleotide

• Length: 399 bp

>T212928 NZ_CP107404:c78392-78243 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8787.73 Da
• Isoelectric Point: 4.2342

>AT212928 WP_102860368.1 NZ_CP081490:1286645-1286878 [Pseudomonas protegens]
METGSVFKSNRSQAIRMPKAVALPDDVTRVDIVAIGRTRIITPAGESWDSWFDEDDRVSADFMNERDQPAEQVREEF

Nucleotide

• Length: 234 bp

>AT212928 NZ_CP107404:78437-78496 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT