Detailed information of TA system TA212926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 108447..110155
• Replicon: chromosome
• Accession: NZ_CP081490
• Organism: Pseudomonas protegens strain PS1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: K5F93_RS00510
• Protein ID: WP_221810217.1
• Coordinates: 109277..110155 (-)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: K5F93_RS00505
• Protein ID: WP_221810216.1
• Coordinates: 108447..109280 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5F93_RS00485 (K5F93_00485)	103580..105076	-	1497	WP_221810213.1	aldehyde dehydrogenase	-
K5F93_RS00490 (K5F93_00490)	105159..105962	-	804	WP_102862343.1	aspartate dehydrogenase	-
K5F93_RS00495 (K5F93_00495)	105973..107655	-	1683	WP_221810214.1	thiamine pyrophosphate-binding protein	-
K5F93_RS00500 (K5F93_00500)	107648..108445	-	798	WP_221810215.1	SDR family oxidoreductase	-
K5F93_RS00505 (K5F93_00505)	108447..109280	-	834	WP_221810216.1	IclR family transcriptional regulator	Antitoxin
K5F93_RS00510 (K5F93_00510)	109277..110155	-	879	WP_221810217.1	alpha/beta fold hydrolase	Toxin
K5F93_RS00515 (K5F93_00515)	110160..110696	-	537	WP_221810218.1	cupin domain-containing protein	-
K5F93_RS00520 (K5F93_00520)	110709..111473	-	765	WP_221812263.1	SDR family oxidoreductase	-
K5F93_RS00525 (K5F93_00525)	111473..112249	-	777	WP_221812264.1	ABC transporter permease	-
K5F93_RS00530 (K5F93_00530)	112279..113280	-	1002	WP_221810219.1	ABC transporter substrate-binding protein	-
K5F93_RS00535 (K5F93_00535)	113320..114156	-	837	WP_260627543.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (45-276)

Antitoxin

• Transcription regulator IclR, C-terminal (134-256)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 31130.76 Da
• Isoelectric Point: 8.0022

>T212926 WP_221810217.1 NZ_CP081490:c110155-109277 [Pseudomonas protegens]
MLPGRSQAASTADLQAQLARDFPLQRVLAAGGQQTLRECGTGPAVVLLHGIGSAAASWLQVAQQLAPKARVIAWDAPGYG
ESSPLESPAPKAEQYATRLMQMLDALDVEQCVLVGHSLGALPALAFARSSQAPRVSRLLLISPTRGYGASERALQRQQVR
QQRLDNLEHLGIAGLADQRTAHMLSANAAPRALAWVHWNMARLNPAGYRQAIELLCGDDLLRHGPPDMPCEVHCGEFDRI
TPAQTCLTLAKALGASFNLIADAGHASPVEQPLVVAGRLAFAIKESMTGRPL

Nucleotide

• Length: 879 bp

>T212926 NZ_CP107403:3611056-3611463 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30390.86 Da
• Isoelectric Point: 6.9725

>AT212926 WP_221810216.1 NZ_CP081490:c109280-108447 [Pseudomonas protegens]
MNDADLLKDPQDKYIVPGLERGLLLLCEFSRSNRTLTAPELARRLALPRSTIFRLLTTLETMGFVTRSGNEYRLGMSVLR
LGFEYLASLELTELGQPLLARLCDRLNYPSNLVVRDGRSIVYVAKVSPPSVFSSAVSVGTRLPAHATVLGRVLLGDLSLA
ELRELYPEEHLEQHSPSTPNTVMALFDMVQADRQRGFVSGEGFFEPSISTVAAPVRDQSGGIVAALGVTIATAQVGHINY
NELLLQVRRSADELSRLLNYNSAAHGGQPRVTALMRD

Nucleotide

• Length: 834 bp

>AT212926 NZ_CP107403:3610809-3611075 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA