Detailed information of TA system TA212921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4351085..4351899
• Replicon: chromosome
• Accession: NZ_CP081489
• Organism: Escherichia coli BL21(DE3)

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K5T46_RS21010
• Protein ID: WP_001054376.1
• Coordinates: 4351085..4351342 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K5T46_RS21015
• Protein ID: WP_001309181.1
• Coordinates: 4351354..4351899 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5T46_RS20990 (4346373)	4346373..4347479	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
K5T46_RS20995 (4347544)	4347544..4348524	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K5T46_RS22005 (4348634)	4348634..4348839	+	206	Protein_4139	HNH endonuclease	-
K5T46_RS21000 (4349107)	4349107..4350347	-	1241	Protein_4140	helicase YjhR	-
K5T46_RS22010 (4350463)	4350463..4350594	+	132	WP_001309182.1	hypothetical protein	-
K5T46_RS21010 (4351085)	4351085..4351342	+	258	WP_001054376.1	YjhX family toxin	Toxin
K5T46_RS21015 (4351354)	4351354..4351899	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K5T46_RS21020 (4351955)	4351955..4352701	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K5T46_RS21025 (4352870)	4352870..4353088	+	219	Protein_4145	hypothetical protein	-
K5T46_RS21030 (4353126)	4353126..4353242	+	117	Protein_4146	VOC family protein	-
K5T46_RS21035 (4353487)	4353487..4354608	+	1122	WP_000010829.1	M42 family metallopeptidase	-
K5T46_RS21040 (4354605)	4354605..4354883	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K5T46_RS21045 (4354895)	4354895..4356208	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T212921 WP_001054376.1 NZ_CP081489:4351085-4351342 [Escherichia coli BL21(DE3)]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T212921 NZ_CP107403:c2175978-2175694 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT212921 WP_001309181.1 NZ_CP081489:4351354-4351899 [Escherichia coli BL21(DE3)]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT212921 NZ_CP107403:c2176210-2175968 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8