Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3313760..3314597 Replicon chromosome
Accession NZ_CP081489
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag K5T46_RS15800 Protein ID WP_000227786.1
Coordinates 3314055..3314597 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag K5T46_RS15795 Protein ID WP_001297137.1
Coordinates 3313760..3314071 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5T46_RS15770 (3308780) 3308780..3309727 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
K5T46_RS15775 (3309749) 3309749..3311740 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
K5T46_RS15780 (3311730) 3311730..3312344 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
K5T46_RS15785 (3312344) 3312344..3312673 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
K5T46_RS15790 (3312685) 3312685..3313575 + 891 WP_000971336.1 heme o synthase -
K5T46_RS15795 (3313760) 3313760..3314071 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
K5T46_RS15800 (3314055) 3314055..3314597 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
K5T46_RS15805 (3314653) 3314653..3315588 - 936 WP_001297127.1 tetratricopeptide repeat protein -
K5T46_RS15810 (3316005) 3316005..3317369 + 1365 WP_001000978.1 MFS transporter -
K5T46_RS15815 (3317497) 3317497..3317988 - 492 WP_001138904.1 nucleotide binding protein YajQ -
K5T46_RS15820 (3318156) 3318156..3319067 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T212916 WP_000227786.1 NZ_CP081489:3314055-3314597 [Escherichia coli BL21(DE3)]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T212916 NZ_CP107401:c6004-5690 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT212916 WP_001297137.1 NZ_CP081489:3313760-3314071 [Escherichia coli BL21(DE3)]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT212916 NZ_CP107401:c6270-5992 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References