212915

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 3214106..3215694 Replicon chromosome
Accession NZ_CP081489
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag K5T46_RS15325 Protein ID WP_001125439.1
Coordinates 3214106..3215428 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag K5T46_RS15330 Protein ID WP_001301023.1
Coordinates 3215428..3215694 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5T46_RS15310 (3209489) 3209489..3212139 - 2651 Protein_3026 fimbrial biogenesis outer membrane usher protein -
K5T46_RS15315 (3212181) 3212181..3212891 - 711 Protein_3027 fimbria/pilus periplasmic chaperone -
K5T46_RS15320 (3213252) 3213252..3213734 - 483 WP_000931227.1 fimbrial protein -
K5T46_RS15325 (3214106) 3214106..3215428 - 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
K5T46_RS15330 (3215428) 3215428..3215694 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K5T46_RS15335 (3215917) 3215917..3217497 - 1581 Protein_3031 autotransporter outer membrane beta-barrel domain-containing protein -
K5T46_RS15340 (3217493) 3217493..3218269 + 777 Protein_3032 histidine kinase -
K5T46_RS15345 (3218281) 3218281..3219303 + 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
K5T46_RS15350 (3219330) 3219330..3220205 + 876 WP_000774188.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
K5T46_RS15355 (3220229) 3220229..3220519 + 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - fimC 3200269..3215694 15425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T212915 WP_001125439.1 NZ_CP081489:c3215428-3214106 [Escherichia coli BL21(DE3)]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T212915 NZ_CP107400:73641-73790 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT212915 WP_001301023.1 NZ_CP081489:c3215694-3215428 [Escherichia coli BL21(DE3)]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT212915 NZ_CP107400:c73596-73537 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References