Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3029463..3030081 Replicon chromosome
Accession NZ_CP081489
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K5T46_RS14460 Protein ID WP_001291435.1
Coordinates 3029463..3029681 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K5T46_RS14465 Protein ID WP_000344800.1
Coordinates 3029707..3030081 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5T46_RS14425 (3024752) 3024752..3025324 + 573 WP_000779826.1 YbaY family lipoprotein -
K5T46_RS14430 (3025355) 3025355..3025666 - 312 WP_000409911.1 MGMT family protein -
K5T46_RS14440 (3026045) 3026045..3026398 + 354 WP_000878140.1 DUF1428 domain-containing protein -
K5T46_RS14445 (3026440) 3026440..3027990 - 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K5T46_RS14450 (3028154) 3028154..3028624 - 471 WP_000136192.1 YlaC family protein -
K5T46_RS14455 (3028740) 3028740..3029291 - 552 WP_000102564.1 maltose O-acetyltransferase -
K5T46_RS14460 (3029463) 3029463..3029681 - 219 WP_001291435.1 HHA domain-containing protein Toxin
K5T46_RS14465 (3029707) 3029707..3030081 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K5T46_RS14470 (3030627) 3030627..3033776 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K5T46_RS14475 (3033799) 3033799..3034992 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T212914 WP_001291435.1 NZ_CP081489:c3029681-3029463 [Escherichia coli BL21(DE3)]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T212914 NZ_CP107400:60552-60968 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT212914 WP_000344800.1 NZ_CP081489:c3030081-3029707 [Escherichia coli BL21(DE3)]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT212914 NZ_CP107400:60325-60555 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References