Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1263209..1263863 Replicon chromosome
Accession NZ_CP081489
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag K5T46_RS06025 Protein ID WP_000244783.1
Coordinates 1263209..1263616 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K5T46_RS06030 Protein ID WP_000354046.1
Coordinates 1263597..1263863 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5T46_RS06005 (1259166) 1259166..1260899 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
K5T46_RS06010 (1260905) 1260905..1261615 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K5T46_RS06015 (1261640) 1261640..1262536 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K5T46_RS06020 (1262648) 1262648..1263169 + 522 WP_001055874.1 flavodoxin FldB -
K5T46_RS06025 (1263209) 1263209..1263616 - 408 WP_000244783.1 protein YgfX Toxin
K5T46_RS06030 (1263597) 1263597..1263863 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K5T46_RS06035 (1264106) 1264106..1265086 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K5T46_RS06040 (1265282) 1265282..1265941 - 660 WP_000250274.1 hemolysin III family protein -
K5T46_RS06045 (1266105) 1266105..1266416 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
K5T46_RS06050 (1266461) 1266461..1267894 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
K5T46_RS06055 (1267951) 1267951..1268694 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T212903 WP_000244783.1 NZ_CP081489:c1263616-1263209 [Escherichia coli BL21(DE3)]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T212903 NZ_CP107398:838539-838946 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT212903 WP_000354046.1 NZ_CP081489:c1263863-1263597 [Escherichia coli BL21(DE3)]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT212903 NZ_CP107398:838292-838558 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References