Detailed information of TA system TA212900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1274497..1275414
• Replicon: chromosome
• Accession: NZ_CP081488
• Organism: Bacillus velezensis strain LZN01

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: K4120_RS06720
• Protein ID: WP_032866111.1
• Coordinates: 1274668..1275414 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: K4120_RS06715
• Protein ID: WP_003154807.1
• Coordinates: 1274497..1274667 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4120_RS06680 (K4120_06680)	1269730..1271352	+	1623	WP_032866113.1	pyocin knob domain-containing protein	-
K4120_RS06685 (K4120_06685)	1271365..1271736	+	372	WP_020955692.1	XkdW family protein	-
K4120_RS06690 (K4120_06690)	1271741..1271938	+	198	WP_020955693.1	XkdX family protein	-
K4120_RS06695 (K4120_06695)	1271995..1272756	+	762	WP_020955694.1	hypothetical protein	-
K4120_RS06700 (K4120_06700)	1272808..1273071	+	264	WP_032866112.1	hemolysin XhlA family protein	-
K4120_RS06705 (K4120_06705)	1273085..1273348	+	264	WP_003154813.1	phage holin	-
K4120_RS06710 (K4120_06710)	1273362..1274240	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
K4120_RS19560	1274275..1274400	-	126	WP_023356844.1	hypothetical protein	-
K4120_RS06715 (K4120_06715)	1274497..1274667	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
K4120_RS06720 (K4120_06720)	1274668..1275414	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K4120_RS06725 (K4120_06725)	1275518..1276516	-	999	WP_020955696.1	inorganic phosphate transporter	-
K4120_RS06730 (K4120_06730)	1276529..1277146	-	618	WP_003154804.1	DUF47 domain-containing protein	-
K4120_RS06735 (K4120_06735)	1277432..1278748	-	1317	WP_007610842.1	amino acid permease	-
K4120_RS06740 (K4120_06740)	1279071..1280021	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T212900 WP_032866111.1 NZ_CP081488:c1275414-1274668 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T212900 NZ_CP107397:1088-1354 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT212900 WP_003154807.1 NZ_CP081488:c1274667-1274497 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT212900 NZ_CP107397:880-1104 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14