Detailed information of TA system TA212898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1699966..1701610
• Replicon: chromosome
• Accession: NZ_CP081487
• Organism: Pasteurella multocida strain Pm64

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V4NI19
• Locus tag: K5Q60_RS08185
• Protein ID: WP_005752891.1
• Coordinates: 1700303..1701610 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V4NAB0
• Locus tag: K5Q60_RS08180
• Protein ID: WP_005752888.1
• Coordinates: 1699966..1700301 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5Q60_RS08155 (K5Q60_08155)	1696044..1696703	+	660	WP_005724855.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
K5Q60_RS08160 (K5Q60_08160)	1696767..1697336	+	570	WP_014326417.1	Der GTPase-activating protein YihI	-
K5Q60_RS08165 (K5Q60_08165)	1697342..1697770	+	429	WP_005719208.1	DUF2489 domain-containing protein	-
K5Q60_RS08170 (K5Q60_08170)	1697794..1699161	+	1368	WP_016504414.1	oxygen-independent coproporphyrinogen III oxidase	-
K5Q60_RS08175 (K5Q60_08175)	1699202..1699870	+	669	WP_014326419.1	nitroreductase family protein	-
K5Q60_RS08180 (K5Q60_08180)	1699966..1700301	-	336	WP_005752888.1	helix-turn-helix transcriptional regulator	Antitoxin
K5Q60_RS08185 (K5Q60_08185)	1700303..1701610	-	1308	WP_005752891.1	HipA domain-containing protein	Toxin
K5Q60_RS08190 (K5Q60_08190)	1701829..1702965	+	1137	WP_075266242.1	anhydro-N-acetylmuramic acid kinase	-
K5Q60_RS08195 (K5Q60_08195)	1703031..1705613	-	2583	WP_014326421.1	DNA mismatch repair protein MutS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-375)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50526.31 Da
• Isoelectric Point: 4.9118

>T212898 WP_005752891.1 NZ_CP081487:c1701610-1700303 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETTLRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Nucleotide

• Length: 1308 bp

>T212898 NZ_CP107395:c91426-91010 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12520.60 Da
• Isoelectric Point: 10.5259

>AT212898 WP_005752888.1 NZ_CP081487:c1700301-1699966 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Nucleotide

• Length: 336 bp

>AT212898 NZ_CP107395:c91653-91423 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: V4NI19

Antitoxin

• AlphaFold DB: V4NAB0