Detailed information of TA system TA212897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1629293..1630937
• Replicon: chromosome
• Accession: NZ_CP081486
• Organism: Pasteurella multocida strain Pm3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V4NI19
• Locus tag: K5E88_RS07670
• Protein ID: WP_005752891.1
• Coordinates: 1629630..1630937 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V4NAB0
• Locus tag: K5E88_RS07665
• Protein ID: WP_005752888.1
• Coordinates: 1629293..1629628 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5E88_RS07640 (K5E88_07640)	1625371..1626030	+	660	WP_005724855.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
K5E88_RS07645 (K5E88_07645)	1626094..1626663	+	570	WP_014326417.1	Der GTPase-activating protein YihI	-
K5E88_RS07650 (K5E88_07650)	1626669..1627097	+	429	WP_005719208.1	DUF2489 domain-containing protein	-
K5E88_RS07655 (K5E88_07655)	1627121..1628488	+	1368	WP_016504414.1	oxygen-independent coproporphyrinogen III oxidase	-
K5E88_RS07660 (K5E88_07660)	1628529..1629197	+	669	WP_014326419.1	nitroreductase family protein	-
K5E88_RS07665 (K5E88_07665)	1629293..1629628	-	336	WP_005752888.1	helix-turn-helix transcriptional regulator	Antitoxin
K5E88_RS07670 (K5E88_07670)	1629630..1630937	-	1308	WP_005752891.1	HipA domain-containing protein	Toxin
K5E88_RS07675 (K5E88_07675)	1631156..1632292	+	1137	WP_075266242.1	anhydro-N-acetylmuramic acid kinase	-
K5E88_RS07680 (K5E88_07680)	1632358..1634940	-	2583	WP_014326421.1	DNA mismatch repair protein MutS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-375)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50526.31 Da
• Isoelectric Point: 4.9118

>T212897 WP_005752891.1 NZ_CP081486:c1630937-1629630 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETTLRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Nucleotide

• Length: 1308 bp

>T212897 NZ_CP107395:c78337-78188 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12520.60 Da
• Isoelectric Point: 10.5259

>AT212897 WP_005752888.1 NZ_CP081486:c1629628-1629293 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Nucleotide

• Length: 336 bp

>AT212897 NZ_CP107395:78382-78441 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: V4NI19

Antitoxin

• AlphaFold DB: V4NAB0