Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 2459..3152 Replicon plasmid unnamed2
Accession NZ_CP081479
Organism Pseudomonas aeruginosa strain P8W

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag K5A80_RS35170 Protein ID WP_003151133.1
Coordinates 2459..2836 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag K5A80_RS35175 Protein ID WP_001172026.1
Coordinates 2817..3152 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5A80_RS35165 (K5A80_35895) 1665..2267 - 603 WP_010465829.1 recombinase family protein -
K5A80_RS35170 (K5A80_35900) 2459..2836 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K5A80_RS35175 (K5A80_35905) 2817..3152 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
K5A80_RS35180 (K5A80_35910) 3167..3502 + 336 WP_000741275.1 hypothetical protein -
K5A80_RS35185 (K5A80_35915) 3526..3852 + 327 WP_000091614.1 hypothetical protein -
K5A80_RS35190 (K5A80_35920) 3868..4209 + 342 WP_025999701.1 hypothetical protein -
K5A80_RS35195 (K5A80_36525) 4416..4616 + 201 WP_242417892.1 hypothetical protein -
K5A80_RS35200 (K5A80_35930) 4734..5789 + 1056 WP_160441112.1 hypothetical protein -
K5A80_RS35205 (K5A80_35935) 6232..6645 + 414 WP_034064646.1 hypothetical protein -
K5A80_RS35210 (K5A80_35940) 7005..7391 + 387 WP_033949800.1 hypothetical protein -
K5A80_RS35215 (K5A80_35945) 7413..7640 + 228 WP_033949799.1 hypothetical protein -
K5A80_RS35220 (K5A80_35950) 7754..8044 + 291 WP_033949796.1 TrfB-related DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..51187 51187
- flank IS/Tn - - 1665..2267 602


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T212895 WP_003151133.1 NZ_CP081479:2459-2836 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T212895 NZ_CP107394:c3999428-3999210 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT212895 WP_001172026.1 NZ_CP081479:2817-3152 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT212895 NZ_CP107394:c3999829-3999455 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References