Detailed information of TA system TA212893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6411276..6411781
• Replicon: chromosome
• Accession: NZ_CP081477
• Organism: Pseudomonas aeruginosa strain P8W

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: K5A80_RS30530
• Protein ID: WP_003083773.1
• Coordinates: 6411276..6411557 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: K5A80_RS30535
• Protein ID: WP_003083775.1
• Coordinates: 6411554..6411781 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5A80_RS30505 (K5A80_31250)	6406527..6407876	+	1350	WP_023910702.1	C4-dicarboxylate transporter DctA	-
K5A80_RS30510 (K5A80_31255)	6407925..6408611	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
K5A80_RS30515 (K5A80_31260)	6408712..6409446	+	735	WP_003117208.1	GntR family transcriptional regulator	-
K5A80_RS30520 (K5A80_31265)	6409626..6410036	+	411	WP_003101225.1	aegerolysin family protein	-
K5A80_RS30525 (K5A80_31270)	6410068..6410976	-	909	WP_003083769.1	LysR family transcriptional regulator	-
K5A80_RS30530 (K5A80_31275)	6411276..6411557	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
K5A80_RS30535 (K5A80_31280)	6411554..6411781	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
K5A80_RS30540 (K5A80_31285)	6411957..6412577	-	621	WP_003101226.1	hypothetical protein	-
K5A80_RS30545 (K5A80_31290)	6412678..6413178	+	501	WP_003112629.1	LEA type 2 family protein	-
K5A80_RS30550 (K5A80_31295)	6413251..6413592	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
K5A80_RS30555 (K5A80_31300)	6413674..6415101	-	1428	WP_003083784.1	GABA permease	-
K5A80_RS30560 (K5A80_31305)	6415270..6416763	-	1494	WP_016263977.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T212893 WP_003083773.1 NZ_CP081477:c6411557-6411276 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T212893 NZ_CP107394:3284274-3284558 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT212893 WP_003083775.1 NZ_CP081477:c6411781-6411554 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT212893 NZ_CP107394:3284042-3284284 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9