Detailed information of TA system TA212891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4984979..4985565
• Replicon: chromosome
• Accession: NZ_CP081477
• Organism: Pseudomonas aeruginosa strain P8W

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: K5A80_RS23920
• Protein ID: WP_003120987.1
• Coordinates: 4985266..4985565 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K5A80_RS23915
• Protein ID: WP_003448662.1
• Coordinates: 4984979..4985269 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5A80_RS23900 (K5A80_24660)	4980563..4982452	+	1890	WP_023101908.1	hypothetical protein	-
K5A80_RS23905 (K5A80_24665)	4982449..4984425	+	1977	WP_031691363.1	DEAD/DEAH box helicase	-
K5A80_RS23910 (K5A80_24670)	4984564..4984908	+	345	WP_023082045.1	hypothetical protein	-
K5A80_RS23915 (K5A80_24675)	4984979..4985269	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
K5A80_RS23920 (K5A80_24680)	4985266..4985565	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5A80_RS23925 (K5A80_24685)	4985767..4986891	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
K5A80_RS23930 (K5A80_24690)	4986891..4988600	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
K5A80_RS23935 (K5A80_24695)	4988604..4989929	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
K5A80_RS23940 (K5A80_24700)	4989919..4990452	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4957096..5037713	80617

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T212891 WP_003120987.1 NZ_CP081477:c4985565-4985266 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T212891 NZ_CP107394:2803906-2804289 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT212891 WP_003448662.1 NZ_CP081477:c4985269-4984979 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT212891 NZ_CP107394:2803664-2803906 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: G8CP73