Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Xre(antitoxin)
Location 996340..997598 Replicon chromosome
Accession NZ_CP081477
Organism Pseudomonas aeruginosa strain P8W

Toxin (Protein)


Gene name - Uniprot ID A0A0H2Z7F3
Locus tag K5A80_RS04800 Protein ID WP_003140635.1
Coordinates 996340..997095 (-) Length 252 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID V6AJU7
Locus tag K5A80_RS04805 Protein ID WP_003086329.1
Coordinates 997230..997598 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5A80_RS04790 (K5A80_05260) 991511..994054 + 2544 WP_079753647.1 acyl-homoserine-lactone acylase QuiP -
K5A80_RS04795 (K5A80_05265) 994209..995570 - 1362 WP_003081943.1 DNA recombination protein RmuC -
K5A80_RS04800 (K5A80_05270) 996340..997095 - 756 WP_003140635.1 RES family NAD+ phosphorylase Toxin
K5A80_RS04805 (K5A80_05275) 997230..997598 - 369 WP_003086329.1 DUF2384 domain-containing protein Antitoxin
K5A80_RS04810 (K5A80_05280) 997671..998957 - 1287 WP_003086327.1 FAD-binding oxidoreductase -
K5A80_RS04815 (K5A80_05285) 999013..1000506 - 1494 WP_003086325.1 aldehyde dehydrogenase family protein -
K5A80_RS04820 (K5A80_05290) 1000681..1001157 + 477 WP_019372052.1 elongation factor GreAB -
K5A80_RS04825 (K5A80_05295) 1001160..1001435 - 276 WP_010792073.1 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(63-207)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-47)


Sequences

Toxin        


Download         Length: 252 a.a.        Molecular weight: 28220.34 Da        Isoelectric Point: 7.7294

>T212887 WP_003140635.1 NZ_CP081477:c997095-996340 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGAPKIFSWEL
FLVDGKLPHPA

Download         Length: 756 bp

>T212887 NZ_CP107394:c712976-712874 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13367.63 Da        Isoelectric Point: 9.9874

>AT212887 WP_003086329.1 NZ_CP081477:c997598-997230 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Download         Length: 369 bp

>AT212887 NZ_CP107394:712867-713012 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z7F3

Antitoxin

Source ID Structure
AlphaFold DB V6AJU7

References