Detailed information of TA system TA212886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 60220..61441
• Replicon: chromosome
• Accession: NZ_CP081477
• Organism: Pseudomonas aeruginosa strain P8W

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: K5A80_RS00410
• Protein ID: WP_003102740.1
• Coordinates: 60863..61441 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K5A80_RS00405
• Protein ID: WP_003088112.1
• Coordinates: 60220..60774 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5A80_RS00380 (K5A80_00365)	55788..56339	+	552	WP_003450159.1	XRE family transcriptional regulator	-
K5A80_RS00385 (K5A80_00370)	56336..56734	-	399	WP_023089053.1	NADH-quinone oxidoreductase subunit A	-
K5A80_RS00390 (K5A80_00375)	56747..57070	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
K5A80_RS00395 (K5A80_00380)	57401..57862	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
K5A80_RS00400 (K5A80_00385)	57867..60062	+	2196	WP_221388741.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
K5A80_RS00405 (K5A80_00390)	60220..60774	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
K5A80_RS00410 (K5A80_00395)	60863..61441	+	579	WP_003102740.1	HD domain-containing protein	Toxin
K5A80_RS00415 (K5A80_00400)	61550..62737	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
K5A80_RS00420 (K5A80_00405)	62727..64898	-	2172	WP_009876279.1	type I secretion system permease/ATPase	-
K5A80_RS00425 (K5A80_00410)	64888..66165	-	1278	WP_003122808.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T212886 WP_003102740.1 NZ_CP081477:60863-61441 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T212886 NZ_CP107393:1088-1354 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT212886 WP_003088112.1 NZ_CP081477:60220-60774 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT212886 NZ_CP107393:880-1104 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07