Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 4669040..4669626 Replicon chromosome
Accession NZ_CP081457
Organism Pseudomonas syringae pv. actinidifoliorum ICMP 18803

Toxin (Protein)


Gene name relE Uniprot ID S6TLE3
Locus tag A237_RS20505 Protein ID WP_017702465.1
Coordinates 4669040..4669348 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S6TPV2
Locus tag A237_RS20510 Protein ID WP_017702466.1
Coordinates 4669345..4669626 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A237_RS20485 (A237_020420) 4664871..4665191 - 321 WP_017702461.1 hypothetical protein -
A237_RS20490 (A237_020425) 4665405..4666616 - 1212 WP_017702462.1 HAMP domain-containing sensor histidine kinase -
A237_RS20495 (A237_020430) 4666751..4667926 - 1176 WP_017702463.1 PAS domain-containing sensor histidine kinase -
A237_RS20500 (A237_020435) 4667916..4668737 - 822 WP_020342226.1 alpha/beta hydrolase -
A237_RS20505 (A237_020440) 4669040..4669348 + 309 WP_017702465.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A237_RS20510 (A237_020445) 4669345..4669626 + 282 WP_017702466.1 putative addiction module antidote protein Antitoxin
A237_RS20515 (A237_020450) 4669715..4670152 - 438 WP_003382543.1 nucleoside deaminase -
A237_RS20520 (A237_020455) 4670186..4670686 - 501 WP_017684511.1 hypothetical protein -
A237_RS20525 (A237_020460) 4670821..4671834 - 1014 WP_020321523.1 ABC transporter substrate-binding protein -
A237_RS20530 (A237_020465) 4671964..4672374 + 411 WP_020341502.1 GNAT family N-acetyltransferase -
A237_RS20535 (A237_020470) 4672399..4672938 - 540 WP_003382539.1 peptide deformylase -
A237_RS20540 (A237_020475) 4672985..4673956 - 972 WP_005767549.1 YihY/virulence factor BrkB family protein -
A237_RS20545 (A237_020480) 4674183..4674374 - 192 WP_017702469.1 CsbD family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-89)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11614.41 Da        Isoelectric Point: 8.4893

>T212882 WP_017702465.1 NZ_CP081457:4669040-4669348 [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MNTLIRTHEFDAWLKGLRDVTARLRILNRIMAAQEGNFGDCEPVGHGISEMRIFVGPGYRVYYTRRAEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEKGDVE

Download         Length: 309 bp

>T212882 NZ_CP107390:c4048143-4047925 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10424.95 Da        Isoelectric Point: 5.1299

>AT212882 WP_017702466.1 NZ_CP081457:4669345-4669626 [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MRELKVFEASDYLNNDEEIAEFLSISLEDPNPDVFLRALQEVAKARGMSQLARDAGINRESLYKALTPGAKPRYDTVLKV
MNALGMKLTAQHV

Download         Length: 282 bp

>AT212882 NZ_CP107390:c4048544-4048170 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M4K279

Antitoxin

Source ID Structure
AlphaFold DB A0A3M4K267

References