Detailed information of TA system TA212877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 1160162..1160776
• Replicon: chromosome
• Accession: NZ_CP081457
• Organism: Pseudomonas syringae pv. actinidifoliorum ICMP 18803

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A3M4KBF3
• Locus tag: A237_RS05310
• Protein ID: WP_017700245.1
• Coordinates: 1160162..1160512 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: S6SUK0
• Locus tag: A237_RS05315
• Protein ID: WP_017685122.1
• Coordinates: 1160528..1160776 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A237_RS05285 (A237_005270)	1155882..1155986	+	105	Protein_1030	IS110 family transposase	-
A237_RS05290 (A237_005275)	1157127..1157903	-	777	WP_158490979.1	YopT-type cysteine protease domain-containing protein	-
A237_RS05295 (A237_005280)	1158227..1159150	-	924	Protein_1032	DEAD/DEAH box helicase	-
A237_RS05300	1159286..1159421	-	136	Protein_1033	prevent-host-death protein	-
A237_RS05305 (A237_005285)	1159507..1159995	+	489	Protein_1034	type III effector	-
A237_RS05310 (A237_005290)	1160162..1160512	-	351	WP_017700245.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A237_RS05315 (A237_005295)	1160528..1160776	-	249	WP_017685122.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
A237_RS05320 (A237_005300)	1161118..1162080	+	963	WP_017700247.1	tyrosine-type recombinase/integrase	-
A237_RS05325 (A237_005305)	1162244..1162495	-	252	WP_017700248.1	hypothetical protein	-
A237_RS05330	1162756..1162969	+	214	Protein_1039	hypothetical protein	-
A237_RS05335 (A237_005315)	1164296..1164790	+	495	WP_017700250.1	hypothetical protein	-
A237_RS05340	1164756..1164927	+	172	Protein_1041	IS5/IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1148325..1176550	28225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12960.81 Da
• Isoelectric Point: 4.2805

>T212877 WP_017700245.1 NZ_CP081457:c1160512-1160162 [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MNTFALRFTEVAQQSLEDQVEHLAVTQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSNGITDIAVLLVLGGKQSVEQTLIRYCLLQLI

Nucleotide

• Length: 351 bp

>T212877 NZ_CP107390:c2399085-2398717 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9389.72 Da
• Isoelectric Point: 9.5019

>AT212877 WP_017685122.1 NZ_CP081457:c1160776-1160528 [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK

Nucleotide

• Length: 249 bp

>AT212877 NZ_CP107390:c2399303-2399082 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A3M4KBF3

Antitoxin

• AlphaFold DB: A0A2V0R8I6