Detailed information of TA system TA212869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2191785..2192314
• Replicon: chromosome
• Accession: NZ_CP081354
• Organism: Staphylococcus aureus strain SQL1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K1X69_RS10965
• Protein ID: WP_000621175.1
• Coordinates: 2191785..2192147 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K1X69_RS10970
• Protein ID: WP_000948331.1
• Coordinates: 2192144..2192314 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1X69_RS10945 (2188763)	2188763..2189533	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
K1X69_RS10950 (2189508)	2189508..2189987	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
K1X69_RS10955 (2189989)	2189989..2190315	-	327	WP_001052491.1	anti-sigma factor antagonist	-
K1X69_RS10960 (2190434)	2190434..2191435	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K1X69_RS10965 (2191785)	2191785..2192147	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K1X69_RS10970 (2192144)	2192144..2192314	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K1X69_RS10975 (2192399)	2192399..2193547	-	1149	WP_001281145.1	alanine racemase	-
K1X69_RS10980 (2193613)	2193613..2193972	-	360	WP_000581200.1	holo-ACP synthase	-
K1X69_RS10985 (2193976)	2193976..2194467	-	492	WP_001205910.1	PH domain-containing protein	-
K1X69_RS10990 (2194454)	2194454..2196037	-	1584	WP_001294626.1	PH domain-containing protein	-
K1X69_RS10995 (2196030)	2196030..2196509	-	480	WP_001287088.1	hypothetical protein	-
K1X69_RS11000 (2196717)	2196717..2197277	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212869 WP_000621175.1 NZ_CP081354:c2192147-2191785 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212869 NZ_CP107384:172652-173095 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212869 WP_000948331.1 NZ_CP081354:c2192314-2192144 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212869 NZ_CP107384:172425-172655 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1